XIONG Yi, LUO Ting-rong, LUI Qi, LI Hua-ming, NAN Song-jian, GUO Jian-gang, DEN Zhao-yang and LAN Bin. Analyzing and Sequencing of the Glycoprotein Gene of Rabies Virus Isolates from Guangxi[J]. Virologica Sinica, 2006, 21(2): 131-135.
Citation: XIONG Yi, LUO Ting-rong, LUI Qi, LI Hua-ming, NAN Song-jian, GUO Jian-gang, DEN Zhao-yang, LAN Bin. Analyzing and Sequencing of the Glycoprotein Gene of Rabies Virus Isolates from Guangxi .VIROLOGICA SINICA, 2006, 21(2) : 131-135.

广西12株狂犬病野毒株的g基因序列测定与分析

  • 通讯作者: 罗廷荣, 
  • 对广西12株狂犬病病毒株g基因全序列进行测序分析,结果显示:广西毒株属于I型,可分为3个群,即Ⅰ群、Ⅱ群和Ⅲ群。GX01、GX08、GX09、GX014、GX091、GX195、GX260、GXLA 8株为Ⅰ群, GX219、GX074、GXBM 3株为Ⅱ群, GXN119株为Ⅲ群。Ⅰ群的广西毒株g基因核苷酸的同源性为97.6%~99.9%,氨基酸同源性在97.7%~100%之间;Ⅱ群的核苷酸的同源性为98.2%~99.0%,氨基酸的同源性在98.5%~99.2%之间。糖蛋白在主要的抗原位点GI、GⅡ区以及与中和抗原有关的36、263、367位氨基酸没有变异,GⅢ区上只有Ⅱ群在332位缬氨酸变异为异亮氨酸,G蛋白上的氨基酸主要在-2、-5、-13、-14、-15、-16、90、96、132、140、156、168、170、204、241、249、253、264、289、332、382、427、436、445、463、474位共26个氨基酸发生了变异,这些氨基酸的变异具有群的特异性。

Analyzing and Sequencing of the Glycoprotein Gene of Rabies Virus Isolates from Guangxi

  • Corresponding author: LUO Ting-rong, 
  • The glycoprotein gene(G) of 12 Rabies virus isolates from different areas in Guangxi were analyzed and sequenced. The results indicated that all rabies virus isolates from Guangxi belong to genotype I and could be divided into 3 groups. GX01, GX08, GX09, GX014, GX091, GX195, GX260 and GXLA strains belong to group Ⅰ. GX219, GX074 and GXBM strains belong to group Ⅱ. GXN119 strain belongs to group Ⅲ. The nucleotide homologies of rabies virus isolates were 97.6% to 99.9% in group Ⅰ and were 98.2% to 99.0% in groupⅡ. The homologies of amino acid sequence were 97.7% to 100% in that the mutations of nucleotide mostly were synonymous mutations, either the main antigenic regions of GI and GⅡ or the main antigenic sites of 36, 263 and 367 amino acid were not any mutative, only the 332 site of valine mutated to isoleucine. The mutations of the deduced amino acid sequence focus on -16, -15, -14, -13, -5, -2, 90, 96, 132, 140, 156, 168, 170, 204, 241, 249, 253, 264, 289, 332, 382, 427, 436, 445, 463 and 474 amino acid on the glycoprotein, all mutations of amino acid has group specificity.

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      沈阳化工大学材料科学与工程学院 沈阳 110142

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    Analyzing and Sequencing of the Glycoprotein Gene of Rabies Virus Isolates from Guangxi

      Corresponding author: LUO Ting-rong,
    • 1. 1. Guangxi General Vererinary Prevention and Quarantine Service , Nanning 530001, China
    • 2. College of Animal Science and Technology, Guangxi University, Nanning 530005, China

    Abstract: The glycoprotein gene(G) of 12 Rabies virus isolates from different areas in Guangxi were analyzed and sequenced. The results indicated that all rabies virus isolates from Guangxi belong to genotype I and could be divided into 3 groups. GX01, GX08, GX09, GX014, GX091, GX195, GX260 and GXLA strains belong to group Ⅰ. GX219, GX074 and GXBM strains belong to group Ⅱ. GXN119 strain belongs to group Ⅲ. The nucleotide homologies of rabies virus isolates were 97.6% to 99.9% in group Ⅰ and were 98.2% to 99.0% in groupⅡ. The homologies of amino acid sequence were 97.7% to 100% in that the mutations of nucleotide mostly were synonymous mutations, either the main antigenic regions of GI and GⅡ or the main antigenic sites of 36, 263 and 367 amino acid were not any mutative, only the 332 site of valine mutated to isoleucine. The mutations of the deduced amino acid sequence focus on -16, -15, -14, -13, -5, -2, 90, 96, 132, 140, 156, 168, 170, 204, 241, 249, 253, 264, 289, 332, 382, 427, 436, 445, 463 and 474 amino acid on the glycoprotein, all mutations of amino acid has group specificity.

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