Highly Soluble Expression of Carboxyl Terminus of Marek’s Disease Virus VP22 in E. coli

CHEN Hong-jun; SONG Cui-ping; QIN Ai-jian*; LIU Yue-long; JIN Wen-jie

PDF
Cite
Share

facebook

twitter

google

linkedin

April 2006

CHEN Hong-jun, SONG Cui-ping, QIN Ai-jian*, LIU Yue-long and JIN Wen-jie. Highly Soluble Expression of Carboxyl Terminus of Marek’s Disease Virus VP22 in E. coli[J]. Virologica Sinica, 2006, 21(2): 169-172.

Citation: CHEN Hong-jun, SONG Cui-ping, QIN Ai-jian*, LIU Yue-long, JIN Wen-jie. Highly Soluble Expression of Carboxyl Terminus of Marek’s Disease Virus VP22 in E. coli .VIROLOGICA SINICA, 2006, 21(2) : 169-172.

MDV-1 VP22 羧基端在大肠杆菌中高效可溶性表达

1.
扬州大学江苏省动物预防医学重点实验室

摘要

VP22是血清I型马立克氏病病毒（MDV-1）的复制和传播必不可少的组分。本研究从MDV-1无致病性的CVI988/Rispens疫苗感染的鸡胚成纤维细胞DNA中扩增得到VP22基因，并在大肠杆菌中表达其C端功能区。SDS-PAGE电泳发现，VP22 C端得到高效可溶性表达，大小为42kDa左右。将获得的阳性条带经切胶免疫和细菌超声波裂解的上清作为抗原免疫6周龄Balb/C小鼠，均能诱导产生特异性抗VP22 C端抗体。通过免疫荧光试验，检测到VP22在感染MDV CEF所有细胞核中呈特异性表达。这一抗体对深入研究VP22蛋白转导功能起到重要的作用。
- 马立克氏病病毒
- , CVI988/Rispens株
- , VP22
- , 大肠杆菌
- , 蛋白转导

Highly Soluble Expression of Carboxyl Terminus of Marek’s Disease Virus VP22 in E. coli

1.
Key Lab of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou, 225009, China

Abstract

Viral protein VP22 is indispensable for the growth and replication of Marek’s disease virus serotype 1(MDV-1). In this study, vp22 gene was amplified from and avirluent CVI988/Rispens strain. Antibodies specific to the carboxyl terminus of VP22 expressed in E. coli were generated by immunizing Balb/C mice 3 times. The antibodies reacted with the complete VP22 expressed in Chick Embryo Fibroblast (CEF) cells infected with CVI988 virus. It is interesting that the VP22 protein could be found in normal CEF cells around MDV plagues. The results suggested that VP22 may be transported among cwlls in MDV infection, which is vital to the studies on functions of VP22.
- Marek’s disease virus (MDV)
- , CVI988/Rispens strain
- , VP22
- , E. coli
- , protein transduction

References
Proportional views

PDF

Article Metrics

Article views(4025) PDF downloads(960) Cited by(0)

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Highly Soluble Expression of Carboxyl Terminus of Marek’s Disease Virus VP22 in E. coli

1. Key Lab of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou, 225009, China

Abstract: Viral protein VP22 is indispensable for the growth and replication of Marek’s disease virus serotype 1(MDV-1). In this study, vp22 gene was amplified from and avirluent CVI988/Rispens strain. Antibodies specific to the carboxyl terminus of VP22 expressed in E. coli were generated by immunizing Balb/C mice 3 times. The antibodies reacted with the complete VP22 expressed in Chick Embryo Fibroblast (CEF) cells infected with CVI988 virus. It is interesting that the VP22 protein could be found in normal CEF cells around MDV plagues. The results suggested that VP22 may be transported among cwlls in MDV infection, which is vital to the studies on functions of VP22.