Molecular Characterization of Hantavirus Zhejiang Isolate ZT10 Strain

WENG Jing-qing; XIE Rong-hui; LU Yi-yu; YAO Ping-ping; LI Min-hong; LI Chan; XU Fang; MAO Hai-yan; ZHU Han-ping; CUI Qing-rong; DONG Ying; ZHU Zhi-yong

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June 2006

WENG Jing-qing, XIE Rong-hui, LU Yi-yu, YAO Ping-ping, LI Min-hong, LI Chan, XU Fang, MAO Hai-yan, ZHU Han-ping, CUI Qing-rong, DONG Ying and ZHU Zhi-yong. Molecular Characterization of Hantavirus Zhejiang Isolate ZT10 Strain[J]. Virologica Sinica, 2006, 21(3): 235-237.

Citation: WENG Jing-qing, XIE Rong-hui, LU Yi-yu, YAO Ping-ping, LI Min-hong, LI Chan, XU Fang, MAO Hai-yan, ZHU Han-ping, CUI Qing-rong, DONG Ying, ZHU Zhi-yong. Molecular Characterization of Hantavirus Zhejiang Isolate ZT10 Strain .VIROLOGICA SINICA, 2006, 21(3) : 235-237.

汉坦病毒东方田鼠分离株ZT10的M基因序列的特性分析

翁景清 ^1*,, ,
谢荣辉 ¹ ,
卢亦愚 ¹ ,
姚苹苹 ¹ ,
李敏红 ¹ ,
李婵 ¹ ,
徐芳 ¹ ,
茅海燕 ¹ ,
朱函坪 ¹ ,
崔庆荣 ² ,
董瑛 ² ,
朱智勇 ¹

1.
1.浙江省疾病预防控制中心, 浙江杭州, 310009
2.
浙江省天台县疾病预防控制中心, 浙江天台, 317200

通讯作者： 翁景清, ;

摘要

分析东方田鼠分离汉坦病毒ZT10株M基因分子特征。提取汉坦病毒ZT10株感染细胞的总RNA，应用逆转录聚合酶链反应(RT-PCR)扩增ZT10株M片段全基因，克隆于T载体并测序，对其进行序列分析。结果显示汉坦病毒ZT10株的基因组M片段长度为3651个核苷酸，编码1133个氨基酸。序列分析表明其为Seoul型汉坦病毒。与八株Seoul型汉坦病毒的M片段同源性为84.0%～96.3%,而与HTN型汉坦病毒的同源性则较低,与从田鼠分离的汉坦病毒（Prospect Hill virus, Tula virus, Khabarovsk virus, Isla vista virus）核苷酸同源性仅为57.5%～60.9%，且与浙江省Seoul型分离株Guo3同源性较低，表明浙江省可能存在着另一Seoul亚型的汉坦病毒。
- 汉坦病毒
- , M基因
- , 序列分析

Molecular Characterization of Hantavirus Zhejiang Isolate ZT10 Strain

1.
1.Zhejiang Provincial Center for Disease Prevention and Control, HangZhou, 310009, China
2.
Zhejiang Provincial TanTai Center for Disease Prevention and Control, Zhejiang TanTai, 317200, China

Corresponding author: WENG Jing-qing,

Abstract

The effects of the M gene on the molecular epidemiological characteristics of the Hantavirus ZT10 strain isolated from M. arvalis was investigated. The gene was amplified by RT-PCR and yielded the expected product of 3651 bp. The amplified fragment was then cloned into the T vector and confirmed by PCR and sequencing. Analysis of the sequence revealed that the ZT10 M segment belongs to seoul virus．The nucleotide sequence identity of this M gene with eight virus strains was 84.0%～96.3%. The identity with Hantanvirus（Prospect Hill virus, Tula virus, Khabarovsk virus, Isla vista virus） isolated from arvalis was 57.5%～60.9%. Also, the identiry with the SEO types and with the Guo3 isoated from ZheJiang province was 84.0%. Seoul virus ZT10 strain is a new specific seoul subtype virus in ZheJiang province.
- Hantavirus
- , Genes
- , Sequence Analysis

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Molecular Characterization of Hantavirus Zhejiang Isolate ZT10 Strain

Corresponding author: WENG Jing-qing,

1. 1.Zhejiang Provincial Center for Disease Prevention and Control, HangZhou, 310009, China
2. Zhejiang Provincial TanTai Center for Disease Prevention and Control, Zhejiang TanTai, 317200, China

Abstract: The effects of the M gene on the molecular epidemiological characteristics of the Hantavirus ZT10 strain isolated from M. arvalis was investigated. The gene was amplified by RT-PCR and yielded the expected product of 3651 bp. The amplified fragment was then cloned into the T vector and confirmed by PCR and sequencing. Analysis of the sequence revealed that the ZT10 M segment belongs to seoul virus．The nucleotide sequence identity of this M gene with eight virus strains was 84.0%～96.3%. The identity with Hantanvirus（Prospect Hill virus, Tula virus, Khabarovsk virus, Isla vista virus） isolated from arvalis was 57.5%～60.9%. Also, the identiry with the SEO types and with the Guo3 isoated from ZheJiang province was 84.0%. Seoul virus ZT10 strain is a new specific seoul subtype virus in ZheJiang province.