Inhibition of Rabies Virus Replication and Infection by Rabies Virus Nucleoprotein Gene siRNA Cocktail

WANG Ji-lin; ZHU Jia-hong*; XU Ge-lin

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June 2006

WANG Ji-lin, ZHU Jia-hong* and XU Ge-lin. Inhibition of Rabies Virus Replication and Infection by Rabies Virus Nucleoprotein Gene siRNA Cocktail[J]. Virologica Sinica, 2006, 21(3): 238-243.

Citation: WANG Ji-lin, ZHU Jia-hong*, XU Ge-lin. Inhibition of Rabies Virus Replication and Infection by Rabies Virus Nucleoprotein Gene siRNA Cocktail .VIROLOGICA SINICA, 2006, 21(3) : 238-243.

用狂犬病毒核蛋白基因小干扰RNA库抑制狂犬病毒复制和感染的研究

1.
武汉生物制品研究所基因工程室，湖北武汉 430060

摘要

采用RNase Ⅲ消化长片段双链RNA的方法，制备了狂犬病毒N基因、P基因和G基因小干扰RNA库（siRNA Cocktail）。将siRNA转染BSR和MNA细胞单层后，采用直接免疫荧光法观察发现，N基因siRNA Cocktail 能够对RV的复制和感染产生明显和稳定的抑制作用，并且通过RT-PCR在转录水平探测到mRNA产量的降低；而P基因或G基因siRNA Cocktail对RV的复制和感染不产生或仅产生弱的抑制作用。这一结果为RNA干扰在RV研究中靶基因的选择及进一步的应用提供了依据。
- 狂犬病毒（RV）
- , 小干扰RNA
- , 复制
- , 抑制

Inhibition of Rabies Virus Replication and Infection by Rabies Virus Nucleoprotein Gene siRNA Cocktail

1.
Wuhan Institute of Biological Products, Wuhan 430060, China

Abstract

Small interference RNA mixtures (siRNA Cocktails ) of Rabies virus (RV) N gene, P gene and G gene were prepared by digesting long double-stranded RNA (dsRNA) with RNase Ⅲ. These siRNA cocktails were transfecteing into BSR or MNA cell monolayers and the direct immunofluores- cence assay (FA) was used to measure the replication and infection of RV on the cell monolayers. We found that the siRNA Cocktails of N gene can obviously and stably inhibit the replication and infection of RV. A descent of mRNA of N gene was also detected by RT-PCR. Under the same situation, the siRNA Cocktails of P gene or G gene showed no or weak inhibition to the replication and infection of RV. All of these results provide a basis for selecting target gene of RV in RNA itreatment and its further application.
- Rabies virus (RV)
- , siRNA Cocktail
- , Replication
- , Inhibition

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Inhibition of Rabies Virus Replication and Infection by Rabies Virus Nucleoprotein Gene siRNA Cocktail

1. Wuhan Institute of Biological Products, Wuhan 430060, China

Abstract: Small interference RNA mixtures (siRNA Cocktails ) of Rabies virus (RV) N gene, P gene and G gene were prepared by digesting long double-stranded RNA (dsRNA) with RNase Ⅲ. These siRNA cocktails were transfecteing into BSR or MNA cell monolayers and the direct immunofluores- cence assay (FA) was used to measure the replication and infection of RV on the cell monolayers. We found that the siRNA Cocktails of N gene can obviously and stably inhibit the replication and infection of RV. A descent of mRNA of N gene was also detected by RT-PCR. Under the same situation, the siRNA Cocktails of P gene or G gene showed no or weak inhibition to the replication and infection of RV. All of these results provide a basis for selecting target gene of RV in RNA itreatment and its further application.