Detection of Apoptosis of the Human Hepatic Carcinoma Cells Induced by Bluetongus Virus by PI and Annexin V/PI Staining Methods

WANG Yan; LIU Chun-xin; YI You-rong; CUI Ye-jian; ZHU Jun; DONG Chang-yuan

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June 2006

WANG Yan, LIU Chun-xin, YI You-rong, CUI Ye-jian, ZHU Jun and DONG Chang-yuan. Detection of Apoptosis of the Human Hepatic Carcinoma Cells Induced by Bluetongus Virus by PI and Annexin V/PI Staining Methods[J]. Virologica Sinica, 2006, 21(3): 253-256.

Citation: WANG Yan, LIU Chun-xin, YI You-rong, CUI Ye-jian, ZHU Jun, DONG Chang-yuan. Detection of Apoptosis of the Human Hepatic Carcinoma Cells Induced by Bluetongus Virus by PI and Annexin V/PI Staining Methods .VIROLOGICA SINICA, 2006, 21(3) : 253-256.

PI法和AnnexinV/PI法检测蓝舌病毒HbC3诱导人肝癌细胞的凋亡

汪艳 ¹ ,
刘春新 ¹ ,
易有荣 ¹ ,
崔冶建 ¹ ,
朱珺 ² ,
董长垣 ^2*,,

1.
1.武汉大学医学结构生物学研究中心, 湖北武汉 430071
2.
武汉大学医学院病毒研究所分子病毒与癌研究室，湖北武汉430071

通讯作者： 董长垣,

摘要

利用流式细胞仪比较PI法和Annexin V/PI法检测不同时间人肝癌细胞感染蓝舌病毒HbC3的凋亡率分析。结果：PI法在 24h、36h、48h的凋亡率分别为10.8 ±3.05、21.7±6.28、28.3±10.6；Annexin V/PI法的凋亡率分别为20.42±3.70、49.3±8.11、79.6±11.5。二种方法及不同时间感染病毒细胞的凋亡率之间有显著差异（P0.01）。证明了Annexin V/PI法能特异地、准确地检出早期凋亡的细胞、继发性坏死的细胞，BTV-HbC3诱导Hep-3B细胞凋亡是致肿瘤细胞病变、死亡的重要表现形式之一。
- Propidium iodide (PI)
- , Annexin V/PI
- , 凋亡
- , 流式细胞仪

Detection of Apoptosis of the Human Hepatic Carcinoma Cells Induced by Bluetongus Virus by PI and Annexin V/PI Staining Methods

1.
1.Center of Medical Structural Biology, Wuhan University School of Medicine Wuhan University, wuhan 430071, China
2.
Laboratory of Virus and Cancer, Institute of Virology, Medical College, Wuhan University, Wuhan 430071,China

Corresponding author: DONG Chang-yuan,

Abstract

Flow cytometry was used to quantify the percentage of apoptotic hepatic Caracimoma cells infected with bluetongue virus HbC3 and stained with propidium iodide (PI) and Annexin V/PI. The percentages of the apoptotic cells measured by PI at 24h, 36h and 48h were 10.8 ±3.05, 21.7±6.28, 28.3±10.6, respectively, and those by Annexin V-PI were 20.42±3.70, 49.3±8.11, 79.6±11.5, respectively. The two staining methods and the rate of apoptotic cells infected by bluetengue virus hours were significantly different (P0.01). The early apoptotic cells and secondary necrosis could be detected by AnnexinV/PI assay with good sensitivity and specificity. The apoptotic cells induced by bluetengue virus was one of the important forms in the pathological changes and cell necrosis.
- Propidium iodide (PI)
- , Annexin V-PI
- , Apoptosis
- , Flow cytometry

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Detection of Apoptosis of the Human Hepatic Carcinoma Cells Induced by Bluetongus Virus by PI and Annexin V/PI Staining Methods

Corresponding author: DONG Chang-yuan,

1. 1.Center of Medical Structural Biology, Wuhan University School of Medicine Wuhan University, wuhan 430071, China
2. Laboratory of Virus and Cancer, Institute of Virology, Medical College, Wuhan University, Wuhan 430071,China

Abstract: Flow cytometry was used to quantify the percentage of apoptotic hepatic Caracimoma cells infected with bluetongue virus HbC3 and stained with propidium iodide (PI) and Annexin V/PI. The percentages of the apoptotic cells measured by PI at 24h, 36h and 48h were 10.8 ±3.05, 21.7±6.28, 28.3±10.6, respectively, and those by Annexin V-PI were 20.42±3.70, 49.3±8.11, 79.6±11.5, respectively. The two staining methods and the rate of apoptotic cells infected by bluetengue virus hours were significantly different (P0.01). The early apoptotic cells and secondary necrosis could be detected by AnnexinV/PI assay with good sensitivity and specificity. The apoptotic cells induced by bluetengue virus was one of the important forms in the pathological changes and cell necrosis.