Sequencing and Analysis of the Genomes of Three Newcastle Disease Viruses Isolated from Guangxi

XIE Zhi-xun; TANG Xiao-fei; DONG Jian-bao; LIU Jia-bo; PANG Yao-shan; DENG Xian-wen; XIE Zhi-qin

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June 2006

XIE Zhi-xun, TANG Xiao-fei, DONG Jian-bao, LIU Jia-bo, PANG Yao-shan, DENG Xian-wen and XIE Zhi-qin. Sequencing and Analysis of the Genomes of Three Newcastle Disease Viruses Isolated from Guangxi[J]. Virologica Sinica, 2006, 21(3): 261-266.

Citation: XIE Zhi-xun, TANG Xiao-fei, DONG Jian-bao, LIU Jia-bo, PANG Yao-shan, DENG Xian-wen, XIE Zhi-qin. Sequencing and Analysis of the Genomes of Three Newcastle Disease Viruses Isolated from Guangxi .VIROLOGICA SINICA, 2006, 21(3) : 261-266.

三株新城疫广西分离株全基因组序列的测定与分析

谢芝勋 ^1*,, ,
唐小飞 ¹ ,
董建宝 ² ,
刘加波 ¹ ,
庞耀珊 ¹ ,
邓显文 ¹ ,
谢志勤 ¹

1.
1. 广西兽医研究所，广西南宁，530001
2.
广西大学动物科学技术学院，广西南宁，530005

通讯作者： 谢芝勋, ;

摘要

根据GenBank上所公布的新城疫病毒（NDV）的全基因组序列，设计了8对引物，运用RT-PCR 方法获取了3株广西地方强毒株GX7/02、GX9/03和GX11/03的全基因组序列，并对其进行了比较分析。此3株病毒的全基因组序列均由15192个碱基组成，与GenBank公布的ZJ1、U.S/Largo/71、Italy/2736/00等7个毒株的全基因组序列长度相同，比LaSota、Clone-30和B1的全基因组序列多出6个核苷酸，此6个核苷酸位于np基因的非编码区内，相对与NDV毒株LaSota、Clone-30和B1序列的1647~1648nt 位。通过序列的比较分析，发现GX7/02、GX9/03和GX11/03与ZJ1毒株的同源性较高，而与LaSota、Clone-30和B1等毒株的同源性较低。
- 新城疫病毒（NDV）
- , 全基因组
- , 测定与分析

Sequencing and Analysis of the Genomes of Three Newcastle Disease Viruses Isolated from Guangxi

1.
1.Guangxi Veterinary Research Institute, Nanning, 530001, China
2.
College of Animal Sciences and Technology, Guangxi University, Nanning, 530005, China

Corresponding author: XIE Zhi-xun,

Abstract

Eight pairs of specific primers were designed and synthesized based on the entire genomic sequence data of Newcastle Disease Virus (NDV) present in GenBank. Complete nucleotide sequences of GX7/02, GX9/03 and GX11/03 strains were acquired by reverse transcription-polymerase reaction. The entire genomic sequences of three NDV strains consisted of 15 192 nt, which was the same in length to 7 NDV ZJ1, U.S/Largo/71, Italy/2736/00 strains, but was 6 nt longer than LaSota, Clone-30 and B1 strains in GenBank. The additional 6 nt motif was located in the non-coding region of nucleoprotein (NP) gene between nucleotides 1647 and 1648 of the entire genomic sequences of NDV LaSota, B1 and Clone-30 strains. The results of identity analysis of nucleotide and amino acid sequences showed that the identities between GX7/02, GX9/03, GX11/03 and ZJ1 strains were higher than that of the three strains of LaSota, B1 and Clone-30.
- Newcastle disease virus
- , Genome
- , Sequencing and analysis

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Sequencing and Analysis of the Genomes of Three Newcastle Disease Viruses Isolated from Guangxi

Corresponding author: XIE Zhi-xun,

1. 1.Guangxi Veterinary Research Institute, Nanning, 530001, China
2. College of Animal Sciences and Technology, Guangxi University, Nanning, 530005, China

Abstract: Eight pairs of specific primers were designed and synthesized based on the entire genomic sequence data of Newcastle Disease Virus (NDV) present in GenBank. Complete nucleotide sequences of GX7/02, GX9/03 and GX11/03 strains were acquired by reverse transcription-polymerase reaction. The entire genomic sequences of three NDV strains consisted of 15 192 nt, which was the same in length to 7 NDV ZJ1, U.S/Largo/71, Italy/2736/00 strains, but was 6 nt longer than LaSota, Clone-30 and B1 strains in GenBank. The additional 6 nt motif was located in the non-coding region of nucleoprotein (NP) gene between nucleotides 1647 and 1648 of the entire genomic sequences of NDV LaSota, B1 and Clone-30 strains. The results of identity analysis of nucleotide and amino acid sequences showed that the identities between GX7/02, GX9/03, GX11/03 and ZJ1 strains were higher than that of the three strains of LaSota, B1 and Clone-30.