设计8对引物分片段扩增桃拉综合征病毒中国分离株ZHZC3全基因组，病毒两末端序列采用末端快速扩增方法（RACE）获取。扩增产物克隆到pMD18-T载体并测序，用DNAstar软件拼接全序列及同源性比较。结果显示ZHZC3全序列除去3′ poly (A)尾，由10202个碱基组成，有两个开放阅读框，分别编码2107和1011个氨基酸的聚蛋白。与美国参考株HI94相比，在编码区没有核苷酸的缺失和插入，但在5′ UTR缺失3个A，两者整体核酸同源性达97.9%。ORF1 中ZHZC3与HI94及巴西株（BLZ01）的核酸同源性分别为97.6%、97.7%，在ORF2中ZHZC3与HI94、BLZ01的核酸同源性则分别为98.3、97%。与国外株ORF2的部分序列比较发现：ZHZC3和中国台湾株均与美国株HI94同源性最高。克隆分析6株TSV中国大陆株主要结构蛋白CP2基因，发现其编码的氨基酸存在三个高变区，中国大陆株更有其独特的氨基酸变异模式，312 (S), 449 (A), 451 (Q) 和468 (H)。表明该病毒的整体变异性不高，但中国的流行株已形成其自己的遗传演变特征。在此基础上，利用生物学软件对CP2蛋白功能域和三维结构进行了预测，为进一步分析CP2蛋白结构与功能关系奠定了基础。ZHZC3株是第一个测定全序列的TSV中国株。

Eight overlapping clones covering the whole viral genome of TSV ZHZC3 isolate were amplified by RT-PCR and the 5′ and 3′ ends were amplified with RACE. The PCR products were cloned into pMD-18T vector and sequenced. The result showed that the genome of ZHZC3 consisted of 10202nt excluding the poly (A) tail, and contained two ORFS (ORFS1-2) which encoded polyproteins of 2107aa and 1011aa. No insertions or deletions were detected in the coding regions while 3nt of A were deleted at 5′ UTR when compared with HI94. The overall nucleotide sequence identity between ZHZC3 and HI94 was 97.6%. Compared with HI94 and BLZ01, the ORF1 of ZHZC3 shared 97.6% and 97.7% nucleotide sequence identity. The ORF2 shared 98.3 and 97% sequence identity at the nucleotide level. Compared with partial sequences of ORF2 from foreign isolates, both ZHZC3 and the Taiwan isolates shared higher nucleotide identity with HI94. Amino acid sequence analysis of six TSV isolates from different regions of mainland China revealed unique aa changes at 312 (S), 449 (A), 451 (Q), and 468 (H) in the highly variable region CP2 of ORF2, indicating that the isolates spread through the South-easten China most closely related to the other isolates with it’s own evolutionary character.