Method for Detecting Tobacco Ringspot Virus by IC-RT-Realtime PCR

YANG Wei-dong; ZHENG Yun; ZHANG Gui-ming; CHEN Zhi-nan; LI Ming-fu; YU Dao-jian; ZHANG Yong-jiang

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June 2006

YANG Wei-dong, ZHENG Yun, ZHANG Gui-ming, CHEN Zhi-nan, LI Ming-fu, YU Dao-jian and ZHANG Yong-jiang. Method for Detecting Tobacco Ringspot Virus by IC-RT-Realtime PCR[J]. Virologica Sinica, 2006, 21(3): 277-280.

Citation: YANG Wei-dong, ZHENG Yun, ZHANG Gui-ming, CHEN Zhi-nan, LI Ming-fu, YU Dao-jian, ZHANG Yong-jiang. Method for Detecting Tobacco Ringspot Virus by IC-RT-Realtime PCR .VIROLOGICA SINICA, 2006, 21(3) : 277-280.

烟草环斑病毒IC-RT-RealtimePCR检测方法研究

杨伟东 ^1,, ,
郑耘 ¹ ,
章桂明 ¹ ,
陈枝楠 ¹ ,
李明福 ² ,
余道坚 ¹ ,
张永江 ²

1.
1. 深圳出入境检验检疫局, 广东深圳518001
2.
中国检验检疫科学院动植物检疫研究所，北京 100029

通讯作者： 杨伟东, ;

摘要

本研究首次应用免疫捕捉反转录实时荧光PCR技术（IC-RT-Realtime PCR）检测烟草环斑病毒，由于综合运用了抗原抗体特异性结合、核酸分子杂交、高灵敏度实时荧光PCR技术的优点，从而使病毒检测在特异性、灵敏度、稳定性等技术指标上比传统的DAS-ELISA方法都有显著提高，解决了烟草环斑病毒检测工作中由于隐症、病毒浓度低、干扰物质存在而影响检测结果的问题。
- 烟草环斑病毒
- , 反转录
- , PCR

Method for Detecting Tobacco Ringspot Virus by IC-RT-Realtime PCR

1.
1.Shenzhen Entry-exit Inspection and Quarantine Bureau, Shenzhen, 518001, China
2.
Institute for Animal & Plant Quarantine of China Academy of Inspection and Quarantine, Beijing 100029, China

Corresponding author: YANG Wei-dong,

Abstract

The technique of immune capture reverse transcription real-time PCR (IC-RT-Realtime PCR) was applied for the first time to detect Tobacco ringspot virus. When detecting virus, it was shown to be advantageous in terms of specificity and sensitivity to integrate several techniques, such as specific bond of antigen and antibody, nucleic acid hybridization and real-time PCR over the traditional technique of DAS-ELISA. Application of the integrated techniques common problems such as masking of symptom, low concentration of virus and disturbing-materials.
- Tobacco ringspot virus
- , Reverse transcription
- , PCR

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Method for Detecting Tobacco Ringspot Virus by IC-RT-Realtime PCR

Corresponding author: YANG Wei-dong,

1. 1.Shenzhen Entry-exit Inspection and Quarantine Bureau, Shenzhen, 518001, China
2. Institute for Animal & Plant Quarantine of China Academy of Inspection and Quarantine, Beijing 100029, China

Abstract: The technique of immune capture reverse transcription real-time PCR (IC-RT-Realtime PCR) was applied for the first time to detect Tobacco ringspot virus. When detecting virus, it was shown to be advantageous in terms of specificity and sensitivity to integrate several techniques, such as specific bond of antigen and antibody, nucleic acid hybridization and real-time PCR over the traditional technique of DAS-ELISA. Application of the integrated techniques common problems such as masking of symptom, low concentration of virus and disturbing-materials.