Cloning and Phylogenetic Analyses of the Nucleoprotein Gene from Coronavirus of Pigeon Origin

QIAN HUA-dong; ZHU JIAN-guo; WU ZHU-li; HUA XIU-guo*

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June 2006

QIAN HUA-dong, ZHU JIAN-guo, WU ZHU-li and HUA XIU-guo*. Cloning and Phylogenetic Analyses of the Nucleoprotein Gene from Coronavirus of Pigeon Origin[J]. Virologica Sinica, 2006, 21(3): 301-303.

Citation: QIAN HUA-dong, ZHU JIAN-guo, WU ZHU-li, HUA XIU-guo*. Cloning and Phylogenetic Analyses of the Nucleoprotein Gene from Coronavirus of Pigeon Origin .VIROLOGICA SINICA, 2006, 21(3) : 301-303.

鸽源冠状病毒核衣壳基因克隆及分子进化分析

1.
上海交通大学农业与生物学院动物科学系 201101

摘要
- 鸽
- , 冠状病毒
- , 核衣壳基因
- , 克隆
- , 序列分析

Cloning and Phylogenetic Analyses of the Nucleoprotein Gene from Coronavirus of Pigeon Origin

1.
School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 201101

Abstract

The nucleoprotein (N) gene of the coronavirus of pigeon origin (PSH) was cloned and sequenced A pair of primers based on the nucleoprotein gene sequences of group 3 coronaviruses was designed and used to amplify the N gene of PSH The PCR product was purified and cloned into the pMD18-T vector. The positive recombinant pTN was used for sequencing. The cloned 1275bp fragment covered all the open reading frame of N gene and potentially encoded 409 amino acids. The results of comparative sequence analysis indicated that the nucleotide sequence of N gene of this strain shared 67.4%～96.6% identity with group 3 coronaviruses, and the predicted amino acid sequence shared 67.4%～97.8％ identity, while only limited identity (41.5%) was observed with group 1 and group 2 coronaviruses. Based on these results, PCoV PSH strain was identified as a member of group 3 coronaviruses.
- Pigeon
- , Coronavirus
- , Nucleoprotein gene
- , Clone
- , Sequence analyses

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Cloning and Phylogenetic Analyses of the Nucleoprotein Gene from Coronavirus of Pigeon Origin

1. School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 201101

Abstract: The nucleoprotein (N) gene of the coronavirus of pigeon origin (PSH) was cloned and sequenced A pair of primers based on the nucleoprotein gene sequences of group 3 coronaviruses was designed and used to amplify the N gene of PSH The PCR product was purified and cloned into the pMD18-T vector. The positive recombinant pTN was used for sequencing. The cloned 1275bp fragment covered all the open reading frame of N gene and potentially encoded 409 amino acids. The results of comparative sequence analysis indicated that the nucleotide sequence of N gene of this strain shared 67.4%～96.6% identity with group 3 coronaviruses, and the predicted amino acid sequence shared 67.4%～97.8％ identity, while only limited identity (41.5%) was observed with group 1 and group 2 coronaviruses. Based on these results, PCoV PSH strain was identified as a member of group 3 coronaviruses.