A New Reovirus Isolate: Biological Characteristics and sequencing of the S4 gene

ZHU Hong; SU Yu-xin; SONG Li-hua; HE Jun; HUANG Ru-tong; MAO Pan-yong; DUAN Qing

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August 2006

ZHU Hong, SU Yu-xin, SONG Li-hua, HE Jun, HUANG Ru-tong, MAO Pan-yong and DUAN Qing. A New Reovirus Isolate: Biological Characteristics and sequencing of the S4 gene[J]. Virologica Sinica, 2006, 21(4): 324-327.

Citation: ZHU Hong, SU Yu-xin, SONG Li-hua, HE Jun, HUANG Ru-tong, MAO Pan-yong, DUAN Qing. A New Reovirus Isolate: Biological Characteristics and sequencing of the S4 gene .VIROLOGICA SINICA, 2006, 21(4) : 324-327.

新分离呼吸道肠道病毒生物学特性和S4片段的序列测定

朱虹 ¹ ,
苏裕心 ¹ ,
宋立华 ¹ ,
何君 ¹ ,
黄如统 ¹ ,
貌盼勇 ^2* ,
端青 ^1*

1.
1.军事医学科学院微生物流行病研究所病原微生物生物安全国家重点实验室，北京 100071
2.
解放军第302医院，北京 100039

摘要

新分离呼吸道肠道病毒（简称呼肠病毒）经过空斑分纯后进行生物学和部分基因序列的鉴定。首先通过BYD株（由端青从北京于某患者标本中分离到的第一株呼肠病毒）对4种传代细胞的敏感性试验，选出L929和LLC-MK2敏感细胞，然后用LLC-MK2细胞进行理化性质试验，血凝试验和形态学鉴定。结果显示该病毒为耐乙醚、不耐酸、不耐热的RNA病毒；对人O型红细胞能产生凝集，滴度达1:32；电镜下可查见细胞胞浆内有球形、直径为70nm～80nm、双层衣壳的病毒颗粒。提取BYD株RNA并进行S4片段序列分析，结果显示该片段的氨基酸序列与已知呼肠病毒1～3型标准株的同源性分别为95%、90% 和95%。以上这些生物学以及S4片断的序列分析结果表明：新分离BYD株病毒符合呼肠病毒科的特性。
- 呼吸道肠道病毒
- , 细胞敏感性
- , 理化性质
- , 形态学
- , 序列分析

A New Reovirus Isolate: Biological Characteristics and sequencing of the S4 gene

ZHU Hong ¹ ,
SU Yu-xin ¹ ,
SONG Li-hua ¹ ,
HE Jun ¹ ,
HUANG Ru-tong ¹ ,
MAO Pan-yong ^2* ,
DUAN Qing ^1*

1.
State Key Laboratory of Pathogen and Biosecurity，Institute of Microbiology and Epidemiology，Academy of Military Medical Sciences，Beijing 100071，China
2.
Hospital of PLA，Beijing 100039

Abstract

The biological Characteristics and partial S4 gene sequence of a new Reovirus isolate was determined. Cell sensitivity testing on four cell lines was conducted by using the new isolate and the data showed that L929 cells and LLC-MK2 cells were highly permissive. The reovirus was resistant to ether, non-resistant to acid and heat in the physicochemical characteristics. It also had hemagglutination activity to human O-erythrocyte, and the titer reached up 1:32. Electron microscopic (EM) examination of LLC-MK2 cells infected with the reovirus revealed characteristic reovirus particles of about 70nm~80nm in diameter. Aggregates of reovirus particles showed in the cytoplasm of L929 cells. The putative S4 gene was amplified by PCR and the product had sequence homology to 3 prototypic strains T1L, T2J and T3D was 84%, 78%, 85%, respectively. All the results suggested that the new reovirus isolate is closely related to the known clusters of Reoviridae.
- Reovirus
- , Cell sensitivity
- , Physicochemical characteristics
- , Virus morphology
- , Sequence analysis

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

A New Reovirus Isolate: Biological Characteristics and sequencing of the S4 gene

1. State Key Laboratory of Pathogen and Biosecurity，Institute of Microbiology and Epidemiology，Academy of Military Medical Sciences，Beijing 100071，China
2. Hospital of PLA，Beijing 100039

Abstract: The biological Characteristics and partial S4 gene sequence of a new Reovirus isolate was determined. Cell sensitivity testing on four cell lines was conducted by using the new isolate and the data showed that L929 cells and LLC-MK2 cells were highly permissive. The reovirus was resistant to ether, non-resistant to acid and heat in the physicochemical characteristics. It also had hemagglutination activity to human O-erythrocyte, and the titer reached up 1:32. Electron microscopic (EM) examination of LLC-MK2 cells infected with the reovirus revealed characteristic reovirus particles of about 70nm~80nm in diameter. Aggregates of reovirus particles showed in the cytoplasm of L929 cells. The putative S4 gene was amplified by PCR and the product had sequence homology to 3 prototypic strains T1L, T2J and T3D was 84%, 78%, 85%, respectively. All the results suggested that the new reovirus isolate is closely related to the known clusters of Reoviridae.