Expression and Functional Characterization of NS5 of Dendrolimus punctatus Cypovirus

ZHANG Wan-ju; ZHAO Shu-ling; ZHANG Xiao-xia; TANG Xian-chun; XIAO Yu-zhou; MEI Chun-lei; SUN Xiu-lian; PENG Hui-ying

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August 2006

ZHANG Wan-ju, ZHAO Shu-ling, ZHANG Xiao-xia, TANG Xian-chun, XIAO Yu-zhou, MEI Chun-lei, SUN Xiu-lian and PENG Hui-ying. Expression and Functional Characterization of NS5 of Dendrolimus punctatus Cypovirus[J]. Virologica Sinica, 2006, 21(4): 401-404.

Citation: ZHANG Wan-ju, ZHAO Shu-ling, ZHANG Xiao-xia, TANG Xian-chun, XIAO Yu-zhou, MEI Chun-lei, SUN Xiu-lian, PENG Hui-ying. Expression and Functional Characterization of NS5 of Dendrolimus punctatus Cypovirus .VIROLOGICA SINICA, 2006, 21(4) : 401-404.

马尾松毛虫质多角体病毒NS5蛋白的表达和功能初步分析

张万菊 ^1,2 ,
赵淑玲 ¹ ,
张小霞 ¹ ,
汤显春 ¹ ,
肖宇宙 ¹ ,
梅春蕾 ¹ ,
孙修炼 ¹ ,
彭辉银 ^1*

1.
1.中国科学院武汉病毒研究所病毒分子生态学实验室，湖北武汉 430071
2.
中国科学院研究生院，北京 100039

摘要

马尾松毛虫质型多角体病毒湖南株第9个片段编码非结构蛋白NS5，利用pET-32a (+) 载体带有组氨酸标记的重组蛋白进行表达，获得纯化的重组蛋白。利用凝胶电泳阻滞检测试验（EMSA）检测蛋白和核酸的结合作用，结果表明NS5蛋白能与病毒基因组dsRNA结合。
- 马尾松毛虫质型多角体病毒（DpCPV）
- , 原核表达
- , 凝胶迁移阻滞试验 (EMSA)

Expression and Functional Characterization of NS5 of Dendrolimus punctatus Cypovirus

1.
1.Virus Molecular Ecology Lab, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China
2.
The Graduate School of Chinese Academy of Sciences, Beijing 100039, China

Abstract

The nucleotide sequence of segment 9 of Dendrolimus punctatus cypovirus (DpCPV) strain Hunan, which encodes the viral non-structural protein (NS5), was tagged with his-tag and expressed by pET32a (+). The recombinant protein was subsequently purified to homogeneity. By using the EMSA, which allows the detection of protein-RNA interactions in the test tube, the possible interactions were tested by the recombinant protein and the double-strand RNA (the genome of DpCPV).
- Dendrolimus punctatus cytoplasmic polyhedrosis virus
- , Prokaryotic expression
- , Electrophore- tic mobility shift assay

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Expression and Functional Characterization of NS5 of Dendrolimus punctatus Cypovirus

1. 1.Virus Molecular Ecology Lab, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China
2. The Graduate School of Chinese Academy of Sciences, Beijing 100039, China

Abstract: The nucleotide sequence of segment 9 of Dendrolimus punctatus cypovirus (DpCPV) strain Hunan, which encodes the viral non-structural protein (NS5), was tagged with his-tag and expressed by pET32a (+). The recombinant protein was subsequently purified to homogeneity. By using the EMSA, which allows the detection of protein-RNA interactions in the test tube, the possible interactions were tested by the recombinant protein and the double-strand RNA (the genome of DpCPV).