DONG Zhi-qiang, ZHANG Zhi, LI Xiao-cheng, CHEN De-kun, ZHANG Yan-xia and HUANG Bao-xu. Optimization of PCR for the Detection of Porcine Circovirus2[J]. Virologica Sinica, 2006, 21(4): 405-407.
Citation: DONG Zhi-qiang, ZHANG Zhi, LI Xiao-cheng, CHEN De-kun, ZHANG Yan-xia, HUANG Bao-xu. Optimization of PCR for the Detection of Porcine Circovirus2 .VIROLOGICA SINICA, 2006, 21(4) : 405-407.

猪圆环病毒PCR检测方法的优化

  • 通讯作者: 张志, 

Optimization of PCR for the Detection of Porcine Circovirus2

  • Corresponding author: ZHANG Zhi, 
  • Three pairs of primers P1/P2, P3/P4 and P5/P6 of porcine circovirus 2 were designed and synthesized to compare the sensitivity and specialization to amplify products of 404bp, 647bp and 1773 bp. Optimum dilution of PK15 cell genome infected with PCV 2 was achieved with 10-6ng, 10-5ng to yield from the three pairs of primers. It was also confirmed that P1/P2 were 68.4% successful in detecting PCV2 in 57 swine samples from the province of Fujian, The other primers, P3/P4, P5/P6, were only 57.9% and 8.7% successful, respectively. We conclude that p1/p2 is the optimum primer pair for surveying incidents of PCV2 in pigs.

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    Optimization of PCR for the Detection of Porcine Circovirus2

      Corresponding author: ZHANG Zhi,
    • 1. 1.North-west Sci-Tech University of Agriculture and Forestry,Yangling 712100, China
    • 2. National Animal Quarantine Institute of Ministry of Agriculture, Qingdao 266032, China

    Abstract: Three pairs of primers P1/P2, P3/P4 and P5/P6 of porcine circovirus 2 were designed and synthesized to compare the sensitivity and specialization to amplify products of 404bp, 647bp and 1773 bp. Optimum dilution of PK15 cell genome infected with PCV 2 was achieved with 10-6ng, 10-5ng to yield from the three pairs of primers. It was also confirmed that P1/P2 were 68.4% successful in detecting PCV2 in 57 swine samples from the province of Fujian, The other primers, P3/P4, P5/P6, were only 57.9% and 8.7% successful, respectively. We conclude that p1/p2 is the optimum primer pair for surveying incidents of PCV2 in pigs.

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