Antiserum Preparation against Rice Dwarf Virus Kunming Isolate and Detection by Immunocapture PCR Tests

PENG Lu-bo; LI Ting-ting; WEI Chun-hong; FANG Qi; DONG Jia-hong; ZHANG Li-zhen; SU Xiao-xia; DING Ming; ZHANG Zhong-kai; LI Yi

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August 2006

PENG Lu-bo, LI Ting-ting, WEI Chun-hong, FANG Qi, DONG Jia-hong, ZHANG Li-zhen, SU Xiao-xia, DING Ming, ZHANG Zhong-kai and LI Yi. Antiserum Preparation against Rice Dwarf Virus Kunming Isolate and Detection by Immunocapture PCR Tests[J]. Virologica Sinica, 2006, 21(4): 408-410.

Citation: PENG Lu-bo, LI Ting-ting, WEI Chun-hong, FANG Qi, DONG Jia-hong, ZHANG Li-zhen, SU Xiao-xia, DING Ming, ZHANG Zhong-kai, LI Yi. Antiserum Preparation against Rice Dwarf Virus Kunming Isolate and Detection by Immunocapture PCR Tests .VIROLOGICA SINICA, 2006, 21(4) : 408-410.

水稻矮缩病毒昆明分离物抗血清制备及免疫捕捉PCR检测

彭潞波 ¹ ,
李婷婷 ¹ ,
魏春红 ² ,
方琦 ¹ ,
董家红 ¹ ,
张丽珍 ¹ ,
苏晓霞 ¹ ,
丁铭 ¹ ,
张仲凯 ^1* ,
李毅 ²

1.
1.云南省农业科学院生物技术及种质资源研究所，云南昆明 650223
2.
北京大学生命科学学院，北京 100871

摘要
- 水稻矮缩病毒
- , 抗血清
- , 间接ELISA
- , 免疫捕捉PCR

Antiserum Preparation against Rice Dwarf Virus Kunming Isolate and Detection by Immunocapture PCR Tests

1.
1.The Biotechnology and Genetic Resources Institute，Yunnan Academy of Agricultural Sciences，Kunming 650223，China
2.
College of Life Science, Peking University, Beijing 100871, China

Abstract

By Using the modified method to extract Rice dwarf virus virions from infected rice leaves, a concentration 3.854mg/mL of purified virus virions reached, and the Rabbits was immunized with purified virions, yielding high titer antiserum; 1:20480 by the indirect enzyme-linked immunosorbent assay (I-ELISA) test. The antiserum successfully detected three rice samples from Kunming, Yuxi and Luliang by I-ELISA.Immunocapture polymerase chain reaction (IC-PCR), respectively was the utilized to show that serologic and molecular methods were successfully used for detection of Rice dwarf virus.
- Rice dwarf virus(RDV)
- , Antiserum
- , I-ELISA
- , IC-PCR

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Antiserum Preparation against Rice Dwarf Virus Kunming Isolate and Detection by Immunocapture PCR Tests

1. 1.The Biotechnology and Genetic Resources Institute，Yunnan Academy of Agricultural Sciences，Kunming 650223，China
2. College of Life Science, Peking University, Beijing 100871, China

Abstract: By Using the modified method to extract Rice dwarf virus virions from infected rice leaves, a concentration 3.854mg/mL of purified virus virions reached, and the Rabbits was immunized with purified virions, yielding high titer antiserum; 1:20480 by the indirect enzyme-linked immunosorbent assay (I-ELISA) test. The antiserum successfully detected three rice samples from Kunming, Yuxi and Luliang by I-ELISA.Immunocapture polymerase chain reaction (IC-PCR), respectively was the utilized to show that serologic and molecular methods were successfully used for detection of Rice dwarf virus.