Apoptosis of the Human Hepatic Carcinoma 3B Cells Induced by Bluetongue Virus HbC3 Strain

WANG Yan; LEI Sen-Lin; GUO Shu-Fang; GAO Qian; DAI Ying; DONG Chang-Yuan

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December 2006

WANG Yan, LEI Sen-Lin, GUO Shu-Fang, GAO Qian, DAI Ying and DONG Chang-Yuan. Apoptosis of the Human Hepatic Carcinoma 3B Cells Induced by Bluetongue Virus HbC3 Strain[J]. Virologica Sinica, 2006, 21(6): 556-559.

Citation: WANG Yan, LEI Sen-Lin, GUO Shu-Fang, GAO Qian, DAI Ying, DONG Chang-Yuan. Apoptosis of the Human Hepatic Carcinoma 3B Cells Induced by Bluetongue Virus HbC3 Strain .VIROLOGICA SINICA, 2006, 21(6) : 556-559.

蓝舌病毒HbC3株诱导人肝癌细胞Hep-3B的凋亡

雷森林 ^1,2 ,
郭淑芳 ,
董长垣 ¹ ,

1.
1. 武汉大学医学院病毒学国家重点实验室, 湖北武汉430071
2.
武汉大学医学结构生物学研究中心, 湖北武汉430071

摘要

采用MTT法检测BTV-HbC3对Hep-3B细胞的增殖抑制作用，流式细胞术检测BTV-HbC3诱导Hep-3B细胞的凋亡情况，透射电镜观察感染BTV-HbC3的Hep-3B细胞超微结构变化。结果表明BTV-HbC3对Hep-3B细胞具有抑制效应，并呈浓度和时间依赖性；BTV-HbC3作用下Hep-3B细胞呈现凋亡特征；BTV-HbC3能有效感染人肝癌细胞株Hep-3B，并在其中有限地复制，同时抑制该细胞增殖，诱导其进入凋亡。本研究证实了蓝舌病毒HbC3株对人肝癌细胞Hep-3B的杀伤及其诱导凋亡作用，结合本室已反复证实该病毒不感染人源正常细胞的事实，提示了该病毒具有抗人肝癌之潜能。
- 蓝舌病毒HbC3(BTV-HbC3)
- , 人肝癌细胞(Hep-3B)
- , 凋亡
- , 溶癌病毒

Apoptosis of the Human Hepatic Carcinoma 3B Cells Induced by Bluetongue Virus HbC3 Strain

1.
1. State Key Laboratory of Virology, Wuhan University School of Medicine, Wuhan 430071, China
2.
Research Center for Medicine and Structural Biology, Wuhan University School of Medicine, Wuhan 430071, China

Corresponding author: DONG Chang-Yuan,

Abstract

When investigating the effect of BTV-HbC3 on the hepatic carcinoma 3B (Hep-3B) cells , we detected a cytopathic effect (CPE) and inhibition activity of Hep-3B cells infected with BTV-HbC3 by using MTT, the apoptosis indicator using flow cytometry (FCM) and microstructures. The results showed that BTV-HbC3 could inhibit the Hep-3B cells proliferation moderately, which depended on the concentration of BTV-HbC3 and time．The human Hepatic carcinoma 3B cells infected by BTV-HbC3 had all the characteristic of apoptosis. Our finding indicated that oncolytic way of BTV-HbC3 was selectively replicated in tumor cells and induced apoptosis. It was further shown in our laboratory that normal human cells couldn’t be infected by this kind of virus suggesting that this virus possessed the potential function of killing the human hepatic carcinoma.
- Bluetongue Virus HbC3
- , Human Hepatic Carcinoma 3B Cells
- , Apoptosis
- , Oncolytic Virus

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Apoptosis of the Human Hepatic Carcinoma 3B Cells Induced by Bluetongue Virus HbC3 Strain

Corresponding author: DONG Chang-Yuan,

1. 1. State Key Laboratory of Virology, Wuhan University School of Medicine, Wuhan 430071, China
2. Research Center for Medicine and Structural Biology, Wuhan University School of Medicine, Wuhan 430071, China

Abstract: When investigating the effect of BTV-HbC3 on the hepatic carcinoma 3B (Hep-3B) cells , we detected a cytopathic effect (CPE) and inhibition activity of Hep-3B cells infected with BTV-HbC3 by using MTT, the apoptosis indicator using flow cytometry (FCM) and microstructures. The results showed that BTV-HbC3 could inhibit the Hep-3B cells proliferation moderately, which depended on the concentration of BTV-HbC3 and time．The human Hepatic carcinoma 3B cells infected by BTV-HbC3 had all the characteristic of apoptosis. Our finding indicated that oncolytic way of BTV-HbC3 was selectively replicated in tumor cells and induced apoptosis. It was further shown in our laboratory that normal human cells couldn’t be infected by this kind of virus suggesting that this virus possessed the potential function of killing the human hepatic carcinoma.