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Transcript size (bp) Harbored SeMNPV genes 5' end sequence 3' end sequence Position in the transcript SeMNPV gene (position in the SeMNPV genome) Position in the transcript Aligned host gene mRNA (position in the host gene) Position in the transcript Aligned host gene mRNA (position in the gene) 1847 nt 106-1634 se5(nt 6190-7713) nt 3-48 B. mori akh2(nt 26-71) nt 1707-1847 No hita 1530 nt 138-1310 se7(nt 9261-10433) nt 1-130 No hit nt 1373-1448 B. mori dh40(nt 3116-3192) 2136 nt 104-2103 se8(nt 12498-14495) nt 12-60 B. mori dh40(nt 1035-998) —b — 978 nt 300-933
nt 55-91
nt 162-229se12(nt 16064-16693)
se11(nt 15817-15852)
se11(nt 15923-16101)nt 5-48 B. mori akh2(nt 27-71) nt 946-978 No hit 1691 nt 348-1511
nt 46-261se43(nt 42696-43856)
se42(nt 42392-42606)nt 1-46 No hit nt 1520-1691 No hit 1118 nt 161-1107
nt 62-70se45(nt 44408-45394)
se44(nt 44305-44312)nt 1-61 No hit — — 1370 nt 488-671
nt 35-489se89(nt 85499-86398)
se88(nt 85088-85799)nt 1-35 No hit — — 1035 nt 407-991
nt 57-323
nt 322-396se124(nt 118809-119391)
se90(nt 86521-86789)
se91(nt 86788-86860)nt 3-50 B. mori adh40(nt 25-71) nt 1007-1035 — 1421 nt 102-1122
nt 1164-1354se126(nt 120802-121788)
se127(nt 121816-122307)nt 1-62 No hit nt 1360-1413 B. mori sifa(nt 462-564) Note: a: The end sequence cannot be aligned to any known sequence in the NCBI's Nucleotide Database.
b: The end sequence is matched to SeMNPV gene.Table 1. The SeMNPV gene-containing full-length transcripts in P8-Se301-C1 cells
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Primer Sequence (5'-3') Genome site (nt) Product size (bp) RT-PCR se5-F GCCTCTGCTATCGTTGCT 6832-6849 858 se5-R CTGATCGGTGGTTTCTCC 7689-7672 se7-F GAGGAGATACGAGGTGATG 9674-9692 753 se7-R TTTCCAAACTTTAGTGCC 10426-10409 se8-F CGCCAAAGACATAGTCCA 12557-12574 1736 se8-R GCGTCAACATTGCCATTA 14292-14275 se12-F TATAGCGTTCTGTTTAGCG 16124-16142 557 se12-R ATTGGATTGGTGCCTTTG 16680-16663 se43-F TCAGCGTCAATAGACTCAT 43066-43084 651 se43-R CGAAGCGATTCATAAAGTA 43716-43698 se45-F ACGACGACTTTACCCAGAA 44523-44541 656 se45-R ATCGGCGACAAACTCAAT 45178-45161 se89-F ACCAACGCCGATTGTCTG 86155-86138 566 se89-R GTGCGGTGGGCATCTTCA 85590-85607 se90-F AGGGACCGTGTCGAAGTA 86765-86748 301 se90-R CTGCCACCGTCAATAGGA 86465-86482 se124-F GGTTGGGTGACGTGATAC 118937-118954 413 se124-R GTCGCTACATTCGTAGTTGT 119349-119330 se126-F TGGGATACTCAAGCCTAAA 121203-121221 533 se126-R TCTCGCTCACCTTCTTATT 121737-121756 RACE-PCR - - GSP-se5-F CAAGAGGAGCCCTGGAAC - - GSP-se5-R GTGGAGGTAGAATACGGC - - GSP-se7-F GAGGAGATACGAGGTGATG - - GSP-se7-R CCGTGATTTCAAACCTTT - - GSP-se8-F CGCCAAAGACATAGTCCA - - GSP-se8-R ATTACCGTTACAACTGCG - - GSP-se12-F AGCGACATACCGTTGCAAGT - - GSP-se12-R GGCGATGTACGCGTTGAAAA - - GSP-se43-F CACCTTCGCCCTCAACAGAT - - GSP-se43-R AGCGCGTACAGAATGCTCTT - - GSP-se45-F TGTCTGCCGCACGAAAAGTA - - GSP-se45-R CTAAACGTCAGTCCGGGCAT - - GSP-se89-F CCGGCCAGTTTGCCAAATAC - - GSP-se89-R TCGTTTCCGCTAACGTCGAA - - GSP-se124-F CCAAAATCCCGACGACAACG - - GSP-se124-R GGTCGCGCATCATCATCAAC - - GSP-se126-F CGTTTCTGCGCGAATCTCTG - - GSP-se126-R TTGATCGCGAGCGAATACGA - - NGSP-se5-F TGATTTCGATGGCCTACCCG - - NGSP-se5-R TTTCCGGTCTGTCATCAGGC - - NGSP-se7-F AGCGCAGAACCGTATGTCAA - - NGSP-se7-R TCTCGTCTCGGTGACCGTAT - - NGSP-se8-F GTGCCGCATGAGCGATAAAG - - NGSP-se8-R TCGCTTTCGAACCTACCCAC - - NGSP-se12-F GAAGCGTTGACGCCGAAAAA - - NGSP-se12-R GCGCGGACGAACTTGAAAAT - - NGSP-se43-F GATTGCAGCCGTTCAAGAGC - - NGSP-se43-R CCCAAGGTGTACGTGTCGAT - - NGSP-se45-F CAGGCGTTGGATTGCATGTG - - NGSP-se45-R TTGACTATACTGTCGGCGGC - - NGSP-se89-F GGCGTCACCTTACGAGACAA - - NGSP-se89-R TGTCGAAGCAGCCGTACATT - - NGSP-se124-F CCGCCGTTAAACAACCATCG - - NGSP-se124-R TCTCCAAGACGACACTCCCA - - NGSP-se126-F GAGCATTCGTTGGTCGAAGC - - NGSP-se126-R AGAACTTGCGCACAAACGTC - - Table S1. Names and sequences of the primers used for the RT-PCR and 5'/3' RACE-PCR
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Cell line Raw reads Quality trimmed Adaptor trimmed rRNA trimmed Clean ratio P8-Se301-C1 54, 569, 296 54, 292, 372 53, 666, 292 52, 784, 058 96.7% Se301 56, 865, 504 56, 639, 360 55, 941, 000 55, 033, 958 96.8% Table S2. Statistical results of the stringent filtering processes
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Cell line Counts Total length (nt) N50 (nt) Mean length N% GC% P8-Se301-C1 116, 048 136, 121, 302 2, 137 1, 173 0.0 37.5 Se301 104, 600 122, 303, 958 2, 145 1, 169 0.0 37.6 Table S3. Statistical results concerning the primary unigenes from P8-Se301-C1 and Se301 cells
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Cell line Counts Total length (nt) N50 (nt) Mean length N% GC% P8-Se301-C1 112, 565 121, 964, 401 1, 824 1, 093 0.0 37.3 Se301 102, 996 109, 124, 144 1, 803 1, 082 0.0 37.4 Table S4. Statistical results concerning the final unigenes from P8-Se301-C1 and Se301 cells
Figure 8 个
Table 5 个
