Figure 1个  Table 2个
    • Example* log of virus dilution Positive units/Total units Cumulative positive (A) Cumulative negative (B) Infected rate (%) A/(A+B)
      Case 1 -5 5/5 9 0 100
      -6 3/5 4 2 66.7
      -7 1/5 1 6 14.3
      -8 0/5 0 11 0
      Case 2 -1 8/8 24 0 100
      -2 7/8 16 1 94.12
      -3 5/8 9 4 69.23
      -4 3/8 4 9 30.77
      -5 1/8 1 16 5.88
      Case 3 -3 6/6 27 0 100
      -4 6/6 21 0 100
      -5 6/6 15 0 100
      -6 5/6 9 1 90
      -7 3/6 4 4 50
      -8 1/6 1 9 10
      aCase 1 was derived from Table A1 in Burleson et al. (1992); case 2 from Table 2.1 in Hierholzer and Killington (1996); and case 3 from Figure 1 in Svensson et al. (1999)

      Table 1.  Sample datasets used to calculate the 50% endpoint using the Reed–Muench method

    • Examplea Method of calculation (TCID50/mL)
      Reed–Muench Spearman–Kärber Modified Kärber (95% CI) Probit regression (95% CI) Logit regression (95% CI)
      Case 1 2.08 × 107 2.00 × 107 2.00 × 107 (5.57 × 106, 7.15 × 107) 2.05 × 107 (2.89 × 106, 1.22 × 108) 1.98 × 107 (2.12 × 106, 1.91 × 108)
      Case 2 3.16 × 104 3.16 × 104 3.16 × 104 (8.42 × 103, 1.19 × 105) 3.40 × 104 (7.54 × 103, 2.01 × 105) 3.42 × 104 (6.77 × 103, 2.31 × 105)
      Case 3 2.00 × 108 2.00 × 108 2.00 × 108 (5.24 × 107, 7.63 × 108) 2.00 × 108 (4.10 × 107, 1.00 × 109) 2.01 × 108 (3.70 × 107, 1.17 × 109)
      aThe examples listed in Table 1 were used to calculate TCID50/mL

      Table 2.  Comparison of five methods for calculating TCID50/mL