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Primers Sequences (5′-3′) Primers Sequences (5′-3′) B∆V1-F AAGGGCGAGATCAAGAACTGCAGCT B'∆V1-F GATATGAAAAACTGCTCTTTCAATCTC B∆V1-R GCACTTCAGGCTCACGCACAGGGG B'∆V1-R AGTGCAATTTAAAGTAACACAGAGTGG B1GS-F CGGCAGCAAGGGCGAGATCAAGAACTGCAGCT 01_AE∆V1-F GAAGTAAGAAACTGTTCTTTTAATGTG B1GS-R CCGCCACCGCACTTCAGGCTCACGCACAGGGG 01_AE∆V1-R GGAACAATTTAAAGTAACACAGAGAGG B2GS-F GGCGGTGGCGGCTCTAAGGGCGAGATCAAGAACTGCAGCT 07_BC∆V1-F GAAATGAAAAATTGCTCTTTCAATACA B2GS-R GCTGCCGCCGCCACCGCACTTCAGGCTCACGCACAGGGG 07_BC∆V1-R ACATTCTAAAGTGACACAGAGTGGGGT B3GS-F GGCGGTGGCGGCTCTCGGCAGCAAGGGCGAGATCAAGAAC 08_BC∆V1-F GGAGTAAAAAATTGCTCTTTCAATGCA B3GS-R GCTGCCGCCGCCACCCCGCCACCGCACTTCAGGCTCACGC 08_BC∆V1-R ACAGTTTAAAGTGACACAGAGTGGGGT B4GS-F GGCGGTGGCGGCTCTGGCGGTGGCGGCTCTAAGGGCGAGA -- -- B4GS-R GCTGCCGCCGCCACCGCTGCCGCCGCCACCGCACTTCAGG -- -- B5GS-F GCGGCAGCGGTGGCGGCGGCAGCGGCGGTGGCGGCTCTGGCGGT -- -- B5GS-R CGCCACCGCACTTCAGGCTCACGCACAGGGGGGTCAGCTTCACG -- -- B6GS-F GGCGGTGGCGGCTCTCGGCAGCAAGGGCGAGATCAAGAAC -- -- B6GS-R GCTGCCGCCGCCACCCCGCCACCGCACTTCAGGCTCACGC -- -- B7GS-F GGCGGTGGCGGCTCTGGCGGTGGCGGCTCTAAGGGCGAGA -- -- B7GS-R GCTGCCGCCGCCACCGCTGCCGCCGCCACCGCACTTCAGG -- -- Table 1. Sequences of primers used in this study
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SIV/HIV-1 SIV/HIV-2 HIV subtypes (group) Historical Contemporary SIVs (49) SIVsmm (33) B 52 180 SIVgsn/mon/mus (8) SIVmac (27) C 43 422 SIVcpz(28) HIV-2 (30) D 10 15 HIV-1 (62)* G 11 26 01_AE 50 143 02_AG 13 22 O 10 8 Note: *For HIV-1, we choose all the representative sequences from each subtype (group) for the analysis. Historical and contemporary sequences were sequences having 15-20-year sampling time intervals and excluded subtypes that did not have a sufficient number of sequences according to our criteria in the methods. Table S1. Env sequences used in this study
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Table 2 个
