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Primers Sequences (5′-3′) BamHI-F2-34-For CGGGATCCATTTTATTGTGTACTATATTAATTTC SacI-F2-76-Rev CGAGCTCTTCGATGACAAAATGCCAAACG BamHI-F2-47-For CGGGATCCTCAAATGAGATTCGTTGAAGTG SacI-F2-123-Rev CGAGCTCGTATATGTATGTTTGCAAATCG BamHI-F2-85-For CGGGATCCCAAGAATAAAAATTTAACCAGTTG SacI-F2-148-Rev CGAGCTCTACTAAGTCGGGATTCATTGC BamHI-F2-129-For CGGGATCCTGAAAAAAATAACGCCATTGATC SacI-F2-173-Rev CGAGCTCTCGTTTGTTGCGACTCGAG SacI-F2-163-Rev CGGAGCTCATCTGTGACTAAAGGTACGGG BamHI-F2-148-For CGGGATCCCAAATCGATGCTAACCGAAAATG Table 1. Primers for the PCR amplification of truncated HaF2 gene. Restriction enzyme sites are underlined.
Figure 6 个
Table 1 个
