Figure 8个  Table 1个
    • Polyamines Nucleic acids/CB[7] Methods Binding constants (Ka, M-1) References
      Putrescine CB[7] Fluorescence titration in 10 mmol/L NH4OAc buffer of pH 6.0 at ambient temperature 3.7 × 105 Hennig et al. (2007)
      Spermidine CB[7] ITC titration in 100 μmol/L CaCl2, NaCl and NaHCO3 at 25 ℃ 2.2 × 105 Da Silva et al. (2014)
      Spermine CB[7] ITC titration in 20 mmol/L PBS buffer of pH 6.0 at 37 ℃ 1.18 × 106 Chen et al. (2017)
      Putrescine DNA Affinity capillary electrophoresis method in 20 mmol/L Tris-HCl buffer of pH 7.0 at 25 ℃ 1.02 × 105 Ouameur and Tajmir-Riahi (2004)
      Spermidine DNA Affinity capillary electrophoresis method in 20 mmol/L Tris-HCl buffer of pH 7.0 at 25 ℃ 1.4 × 105 Ouameur and Tajmir-Riahi (2004)
      Spermine DNA Affinity capillary electrophoresis method in 20 mmol/L Tris-HCl buffer of pH 7.0 at 25 ℃ 2.3 × 105 Ouameur and Tajmir-Riahi (2004)
      Spermine DNA Gel filtration method in 100 mmol/L Tris-HCl, 2 mmol/ L Mg2+ and 100 mmol/L K+ buffer of pH 7.5 at 4 ℃ 103×105 Igarashi et al. (1982)
      Spermidine 16S rRNA Gel filtration method in 100 mmol/L Tris-HCl, 2 mmol/ L Mg2+ and 100 mmol/L K+ buffer of pH 7.5 at 4 ℃ 2.2 × 103 Igarashi et al. (1982)
      Spermine 16S rRNA Gel filtration method in 100 mmol/L Tris-HCl, 2 mmol/ L Mg2+ and 100 mmol/L K+ buffer of pH 7.5 at 4 ℃ 1.8 × 104 Igarashi et al. (1982)

      Table 1.  Binding affinities of polyamines and CB[7] or nucleic acids.