Figure 5个  Table 3个
    • Primer Sequence 5'-3' Positions Amplicon size (bp)
      F1 AGCCTTTTGCGGTGACGATAGT 4490-4511 242
      R1 ACTCCTCCAAGTGTTCCCAATC 4710-4731
      F2 GCATCGCATCTGTTCAAGGGAC 405-426 271
      R2 GTAGCGCAATGGCGTACACTCT 654-675
      F3 TGTGTCGGTGTGTATTGTTT 5379-5398 204
      R3 CTACTATTTTTTCCCTTTGCG 5563-5582
      F4 TAAAGCACAACCCAAGCAA 3977-3995 117
      R4 TCACTAAACAACGGGCAAA 4074-4093

      Table 1.  Primers sequences for detecting TMV

    • Centrifugal Force (g) PEG6000 concentration (%)
      1 10 20 30
      3000 0.70%a
      (0.05%)b       		13.10%
      (0.05%)      		 11.63%
      (0.06%)      		 8.89%
      (0.08%)
      4000 4.07%
      (0.05%)      		 16.66%
      (0.05%)      		 21.02%
      (0.06%)      		 17.50%
      (0.08%)
      5000 8.66%
      (0.12%)      		 24.34%
      (0.20%)      		 50.36%
      (0.16%)      		 38.52%
      (0.05%)
      6000 9.89%
      (0.03%)      		 44.79%
      (0.11%)      		 90.95%
      (0.16%)      		 62.02%
      (0.05%)
      7000 8.68%
      (0.26%)      		 32.82%
      (0.22%)      		 41.88%
      (0.13%)      		 11.40%
      (0.12%)
        Orthogonal design tests were carried out at room temperature, and the pH of standard water was ~7.2.aWithin columns indicates recovery efficiency, calculated according to the equation E (%) = (viral copy of recovered TMV/viral copy of initial seeded TMV) ×100; bwithin columns signifies standard error.

      Table 2.  Orthogonal design tests for method optimization

    • Sample Real-time PCR RT-PCR ELISA
      Location Number Positive sample Positive sample Positive sample
      Long County 8 5 4 1
      Xunyang County 9 7 5 2
      Fu County 6 3 2 1
      Nanzheng County 5 2 1 1
      Total 28 17 12 5

      Table 3.  Clinical detection results with real-time PCR, RT-PCR and ELISA