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Primer name Sequences nt Primers used for amplification of pGF vectors XAF-1 GCTCGTAGATCGTCATGGTTTGTTTCTCACCTGGTAGTTCgcggccgcgatcctctagagtcgacctg 68 XAR-1 AAGCTTAGGTACTTCTGGTAACGGTTTACGAGTGACGACAgcggccgccgggtaccgagctcgaattc 68 XBF-1 CCTCCTGGTCGTACAGCAACACCAACTAGACGACTACCGCgcggccgcgatcctctagagtcgacctg 68 XBR-1 GTGACGGAGCGTCGTAGAGAACGGTAGCTCGTGACGGTTGgcggccgccgggtaccgagctcgaattc 68 XCF-1 AAAGCGAAGACTTGCCCGAACTCCCGTCACTTGAAGACACgcggccgcgatcctctagagtcgacctg 68 XCR-1 GCTGGCCGACCTGCTCGACGCTATCGAAGACGACGCACAGgcggccgccgggtaccgagctcgaattc 68 Primers used for amplification of fragments A1-A4, B1-B4, C1-C2 AF1 GAACTACCAGGTGAGAAACAAACCATG 27 AR1 ACCATCGTTACAGGTTTTAACATCGG 26 AF2 TGACTTGGCACGGTGTACTAGGTCAC 26 AR2 GCTGCTACGTTTGTAGACCTAACCTATAAG 30 AF3 TCTGATAGCATCATCAAAGCCGTTAC 26 AR3 ACCTATGTTGTCTGGTCGGTCGTTT 25 AF4 CTTTGATGATGACAGCCGTAGGTTT 25 AR4 TGTCGTCACTCGTAAACCGTTACCAG 26 BF1 GCGGTAGTCGTCTAGTTGGTGTTG 24 BR1 CACAGTGGAACCAACAACTACTAGAAGC 28 BF2 GGTCACGTAAGCTTGAGATCACCTCT 26 BR2 GTACTTCGACAACGAAGATGTGCTACC 27 BF3 CTACTAAGTCATCTACATCCTGACCAGAAGC 31 BR3 GAAAAGCAAGCACAACGAGCTAAGC 25 BF4 ACAGCTTGTTGTCTTTTTCTTTCACGTATC 30 BR4 CAACCGTCACGAGCTACCGTTC 22 CF1 GTGTCTTCAAGTGACGGGAGTTCG 24 CR1 ATGCTTGTCGTAGACCCTGCTGTG 24 CF2 TCGTTGTCGTAACCACCTACGGTC 24 CR2 CTGTGCGTCGTCTTCGATAGCGTCGAG 27 Table S1. Primers used for PCR amplification of pGF vectors and fragments
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Primer name Sequences nt FV CCCAGGCTTTACACTTTATGCTTC 24 RA1 GCAACCGACACACAGCCTCTT 21 FA1 TACACGGGTGGGGTGTTAGTACC 23 RA2 CTTCTGCTTTTGAAAGACCTCCTCT 25 FA2 CTGGGTCGTACATGATAGCTGCTG 24 RA3 TGGTCACTTACAACGTATCATCCCT 25 FA3 CTTGTTCACCTCGTCTACTAACCG 24 RA4 AGTGCTACTAACTGGGTTGTCGATG 25 FA4 GTTCTACAGAAGGACGTGGTTGTGT 25 RB1 GTAACAACAGCTACACTGTGGCTG 24 FB1 CTGTGCTCGTTGTCGTTCTACTG 23 RB2 AGAAGGGTGAGATACAACGCTTTG 24 FB2 CACAGAGTTTCCACGAAGCCTC 22 RB3 CTTCAAGTAGTGCCTTACGACAACG 25 FB3 CCTTGTCGTTTACTGACGTTACCAC 25 RB4 GTTATCAAACGTGCGTCTTACCTCA 25 FB4 TTTTCCCAGAGTTTACGCACCTC 23 RC1 TAGTTCCTATCGGAAGCGACCAT 23 FC1 GAAGTTGTACACTTTGGCGGGAG 23 RC2 CTATCACTACAACCCAGTAGCCAACA 26 FC2 GGGCAAGGCTCTTAGGGTCTTCATTC 26 RV GTGAAATACCACACAGATGCGTAAG 25 Table S2. Primers designed to amplify the sequences across the adjacent DNA fragments
Figure 5 个
Table 2 个
