Figure 3个  Table 2个
    • Case A Case B Case C
      Personal information
      Age and gender 52, female 59, male 67, male
      Occupation Farmer Farmer Farmer
      Admission day May 13, 2015 (the 4th day after illness onset) June 21, 2015 (the 5th day after illness onset) April 7, 2016 (the 5th day after illness onset)
      Location Macheng Guangshui Macheng
      Outcome Survived Fatal Survived
      Clinical manifestation on admission
      Fever Headache Fatigue Myalgia Yes♮ Yes Yes Yes Yes (38 ℃) Yes Yes Yes Yes (39.5 ℃) Yes Yes Yes
      Gastrointestinal symptoms
      Diarrhea Diarrhea Abdominal pain Nausea Vomiting Diarrhea Abdominal pain
      Respirotory symptoms
      Rales in lung Rales in lung Dyspnea Rales in lung
      Neurologic symptoms
      Headache Headache Blurred mind Limb tremor Headache Blurred mind
      Blood counts
      WBC (× 109/L) 3.37↓ 1.97↓ 2.16↓
      PLT (× 109/L) 40↓ 16↓ 21↓
      Urine routine test
      Proteinuria +++ ++ +++
      Hematuria +++ +++ +++
      Serum biochemistry
      ALT (U/L) 75↑ 91↑ 78↑
      AST (U/L) 353↑ 291↑ 291↑
      LDH (U/L) 4309↑ 4602↑ 192
      CK (U/L) 1502↑ 1096↑ 666↑
      CK-MB (U/L) 9.5 3.7 0.9
      Laboratory tests
      qRT-PCR (TCID50/mL) 9.74 × 104 3.41 × 105 4.99 × 105
      Virus isolation HBMC16 HBGS13 HBMC5
      Note: WBC, white blood cells; PLT, platelet count; ALT, alanine aminotransferase; AST, aspartate aminotransferase; CK, creatine kinase; LDH, lactate dehydrogenase. CK-MB, creatine kinase isoenzyme. aThe accurate body temperature of the patient is uncertain. Clinical parameters out of the normal range are shown in bold numbers with up or down arrows.

      Table 1.  Personal information, clinical features, and laboratory tests for SFTS patients

    • Primers Sequences (5'-3') Locations of primers
      S segment
      S1F acacaaagacccccttcatttrg 1-23
      S1R ACACAAAGAACCCCCAAAAAAGGA 1722-1744
      M segment
      M1F acacaragacggccaacaatga 1-22
      M1R TCTCCCAGTTGTGAYGCATTCCTTC 1767-1791
      M2F GGCAACCAWGATGATGTTAGGAT 1597-1619
      M2R ACACAAAGACCGGCCAACACT 3358-3378
      L segment
      L1 F acacaragacgcccagatgrac 1-22
      L1 R GAGACCACTGRACCACATTRCTG 1549-1571
      L2F GTGTCAATCTTGTTGGAAAARGCAT 1463-1487
      L2R GAGCTTYGAGACGAAATARGAC 3313-3314
      L3F GGTTGAAGTCAGCCCGYAGTCT 2913-2934
      L3R ATTCTRACTACTTGGCTTATGGTGG 4800-4824
      L4-F TTAGGGAGAGAAACATTGTCAGGAG 4485-4509
      L4-R acacaaagaccgcccagatctta 6346-6368

      Table S1.  Primers used to sequence the complete genome of SFTSV isolates