Figure 5个  Table 2个
    • Vaccine group gE (μg) CpG (μg) Poly I:C (μg)
      In Out BW006 2395
      ① gE 10
      ② gE + CpG + Poly I:C 10 5 5 25
      ③ gE + Alum 10
      ④ gE + Freund's 10
      ⑤ DOTAP-IN 10 5 5 25
      ⑥ DOTAP-HALF 5 5 5 5 25
      ⑦ DOTAP-OUT 10 5 5 25
      ⑧ PLGA 10 5 5 25
      ⑨ DOPC 10 5 5 25
      ①-④ are mixtures, and ⑤-⑨ are nanoparticles all contain gE+CpG+Poly I:C. For DOTAP-IN (⑤), gE only exists in the inner water phase of the (water/oil/water) emulsions; for DOTAP-OUT (⑦), gE only exists in the outer water phase; for DOTAP-HALF (⑥), gE exists in both water phases.

      Table 1.  Composition of each dose of the designed vaccines.

    • Vaccine group gE (mg) CpG (mg) Poly I:C (mg)
      In Out BW006 2395
      ⑤ DOTAP-IN 0.53 0.19 0.19 0.98
      ⑥ DOTAP-HALF 0.32 0.30 0.30 0.30 1.53
      ⑦ DOTAP-OUT 0.61 0.27 0.27 1.38
      ⑧ PLGA 0.55 0.21 0.21 1.07
      ⑨ DOPC 0.5 0.17 0.17 0.86

      Table 2.  Composition of 50 doses of the nanoparticle vaccines.