• Belouzard, S., Millet, J.K., Licitra, B.N.,Whittaker, G.R., 2012. Mechanisms of coronavirus cell entry mediated by the viral spike protein. Viruses, 4, 1011-1033.

  • Braga, L., Ali, H., Secco, I., Chiavacci, E., Neves, G., Goldhill, D., Penn, R., Jimenez-Guardeño, J.M., Ortega-Prieto, A.M., Bussani, R., Cannatà, A., Rizzari, G., Collesi, C., Schneider, E., Arosio, D., Shah, A.M., Barclay, W.S., Malim, M.H., Burrone, J.,Giacca, M., 2021. Drugs that inhibit TMEM16 proteins block SARS-CoV-2 spike-induced syncytia. Nature, 594, 88-93.

  • Burkard, C., Verheije, M.H., Wicht, O., Van Kasteren, S.I., Van Kuppeveld, F.J., Haagmans, B.L., Pelkmans, L., Rottier, P.J., Bosch, B.J.,De Haan, C.A., 2014. Coronavirus cell entry occurs through the endo-/lysosomal pathway in a proteolysis-dependent manner. PLoS Pathog, 10, e1004502.

  • Cao, L., Ge, X., Gao, Y., Ren, Y., Ren, X.,Li, G., 2015. Porcine epidemic diarrhea virus infection induces NF-κB activation through the TLR2, TLR3 and TLR9 pathways in porcine intestinal epithelial cells. J Gen Virol, 96, 1757-1767.

  • Chang, Z., Wang, Y., Zhou, X.,Long, J.E., 2018. STAT3 roles in viral infection:antiviral or proviral? Future Virol, 13, 557-574.

  • Chen, W., Mook, R.A., Jr., Premont, R.T.,Wang, J., 2018. Niclosamide:Beyond an antihelminthic drug. Cell Signal, 41, 89-96.

  • De Almeida, L., Da Silva, A.L.N., Rodrigues, T.S., Oliveira, S., Ishimoto, A.Y., Seribelli, A.A., Becerra, A., Andrade, W.A., Ataide, M.A., Caetano, C.C.S., De Sá, K.S.G., Pelisson, N., Martins, R.B., De Paula Souza, J., Arruda, E., Batah, S.S., Castro, R., Frantz, F.G., Cunha, F.Q., Cunha, T.M., Fabro, A.T., Cunha, L.D., Louzada-Junior, P., De Oliveira, R.D.R.,Zamboni, D.S., 2022. Identification of immunomodulatory drugs that inhibit multiple inflammasomes and impair SARS-CoV-2 infection. Sci Adv, 8, eabo5400.

  • Dong, S., Yu, R., Wang, X., Chen, B., Si, F., Zhou, J., Xie, C., Li, Z.,Zhang, D., 2022. Bis-Benzylisoquinoline Alkaloids Inhibit Porcine Epidemic Diarrhea Virus In Vitro and In Vivo. Viruses, 14.

  • Eaton, A.F., Merkulova, M.,Brown, D., 2021. The H(+)-ATPase (V-ATPase):from proton pump to signaling complex in health and disease. Am J Physiol Cell Physiol, 320, C392-c414.

  • Gassen, N.C., Papies, J., Bajaj, T., Emanuel, J., Dethloff, F., Chua, R.L., Trimpert, J., Heinemann, N., Niemeyer, C., Weege, F., Hönzke, K., Aschman, T., Heinz, D.E., Weckmann, K., Ebert, T., Zellner, A., Lennarz, M., Wyler, E., Schroeder, S., Richter, A., Niemeyer, D., Hoffmann, K., Meyer, T.F., Heppner, F.L., Corman, V.M., Landthaler, M., Hocke, A.C., Morkel, M., Osterrieder, N., Conrad, C., Eils, R., Radbruch, H., Giavalisco, P., Drosten, C.,Müller, M.A., 2021. SARS-CoV-2-mediated dysregulation of metabolism and autophagy uncovers host-targeting antivirals. Nat Commun, 12, 3818.

  • Gerdts, V.,Zakhartchouk, A., 2017. Vaccines for porcine epidemic diarrhea virus and other swine coronaviruses. Vet Microbiol, 206, 45-51.

  • Harrison, S.C., 2008. Viral membrane fusion. Nat Struct Mol Biol, 15, 690-698.

  • Hemmat, N., Asadzadeh, Z., Ahangar, N.K., Alemohammad, H., Najafzadeh, B., Derakhshani, A., Baghbanzadeh, A., Baghi, H.B., Javadrashid, D., Najafi, S., Ar Gouilh, M.,Baradaran, B., 2021. The roles of signaling pathways in SARS-CoV-2 infection; lessons learned from SARS-CoV and MERS-CoV. Arch Virol, 166, 675-696.

  • Huang, H., Li, Y., Li, D., Wang, L., Jiao, W., Bai, Y.,Zhang, G., 2022. The tyrosine phosphatase PTPN14 inhibits the activation of STAT3 in PEDV infected Vero cells. Vet Microbiol, 267, 109391.

  • Huang, Y.W., Dickerman, A.W., Piñeyro, P., Li, L., Fang, L., Kiehne, R., Opriessnig, T.,Meng, X.J., 2013. Origin, evolution, and genotyping of emergent porcine epidemic diarrhea virus strains in the United States. mBio, 4, e00737-00713.

  • Huynh, J., Chand, A., Gough, D.,Ernst, M., 2019. Therapeutically exploiting STAT3 activity in cancer-using tissue repair as a road map. Nat Rev Cancer, 19, 82-96.

  • Icho, S., Rujas, E., Muthuraman, K., Tam, J., Liang, H., Landreth, S., Liao, M., Falzarano, D., Julien, J.P.,Melnyk, R.A., 2022. Dual Inhibition of Vacuolar-ATPase and TMPRSS2 Is Required for Complete Blockade of SARS-CoV-2 Entry into Cells. Antimicrob Agents Chemother, 66, e0043922.

  • Jeon, S., Ko, M., Lee, J., Choi, I., Byun, S.Y., Park, S., Shum, D.,Kim, S., 2020. Identification of Antiviral Drug Candidates against SARS-CoV-2 from FDA-Approved Drugs. Antimicrob Agents Chemother, 64.

  • Jitobaom, K., Boonarkart, C., Manopwisedjaroen, S., Punyadee, N., Borwornpinyo, S., Thitithanyanont, A., Avirutnan, P., Auewarakul, P.J.B.P.,Toxicology, 2022. Synergistic anti-SARS-CoV-2 activity of repurposed anti-parasitic drug combinations. 23, 1-13.

  • Jung, E., Nam, S., Oh, H., Jun, S., Ro, H.J., Kim, B., Kim, M.,Go, Y.Y., 2019. Neutralization of Acidic Intracellular Vesicles by Niclosamide Inhibits Multiple Steps of the Dengue Virus Life Cycle In Vitro. Sci Rep, 9, 8682.

  • Jung, K., Saif, L.J.,Wang, Q., 2020. Porcine epidemic diarrhea virus (PEDV):An update on etiology, transmission, pathogenesis, and prevention and control. Virus Res, 286, 198045.

  • Jurgeit, A., Mcdowell, R., Moese, S., Meldrum, E., Schwendener, R.,Greber, U.F., 2012. Niclosamide is a proton carrier and targets acidic endosomes with broad antiviral effects. PLoS Pathog, 8, e1002976.

  • Kadri, H., Lambourne, O.A.,Mehellou, Y., 2018. Niclosamide, a Drug with Many (Re)purposes. ChemMedChem, 13, 1088-1091.

  • Kao, J.C., Huangfu, W.C., Tsai, T.T., Ho, M.R., Jhan, M.K., Shen, T.J., Tseng, P.C., Wang, Y.T.,Lin, C.F., 2018. The antiparasitic drug niclosamide inhibits dengue virus infection by interfering with endosomal acidification independent of mTOR. PLoS Negl Trop Dis, 12, e0006715.

  • Kumar, N., Sharma, S., Kumar, R., Tripathi, B.N., Barua, S., Ly, H.,Rouse, B.T., 2020. Host-Directed Antiviral Therapy. Clin Microbiol Rev, 33.

  • Li, W., Li, H., Liu, Y., Pan, Y., Deng, F., Song, Y., Tang, X.,He, Q., 2012. New variants of porcine epidemic diarrhea virus, China, 2011. Emerg Infect Dis, 18, 1350-1353.

  • Li, X., Sun, J., Prinz, R.A., Liu, X.,Xu, X., 2020. Inhibition of porcine epidemic diarrhea virus (PEDV) replication by A77 1726 through targeting JAK and Src tyrosine kinases. Virology, 551, 75-83.

  • Li, Y., Li, P., He, Q., Zhang, R., Li, Y., Kamar, N., Peppelenbosch, M.P., De Man, R.A., Wang, L.,Pan, Q., 2022. Niclosamide inhibits hepatitis E virus through suppression of NF-kappaB signalling. Antiviral Res, 197, 105228.

  • Li, Y., Li, P.K., Roberts, M.J., Arend, R.C., Samant, R.S.,Buchsbaum, D.J., 2014. Multi-targeted therapy of cancer by niclosamide:A new application for an old drug. Cancer Lett, 349, 8-14.

  • Lin, H., Li, B., Liu, M., Zhou, H., He, K.,Fan, H., 2020. Nonstructural protein 6 of porcine epidemic diarrhea virus induces autophagy to promote viral replication via the PI3K/Akt/mTOR axis. Vet Microbiol, 244, 108684.

  • Liu, B., Palmfeldt, J., Lin, L., Colaço, A., Clemmensen, K.K.B., Huang, J., Xu, F., Liu, X., Maeda, K., Luo, Y.,Jäättelä, M., 2018. STAT3 associates with vacuolar H(+)-ATPase and regulates cytosolic and lysosomal pH. Cell Res, 28, 996-1012.

  • Liu, C., Ma, Y., Yang, Y., Zheng, Y., Shang, J., Zhou, Y., Jiang, S., Du, L., Li, J.,Li, F., 2016. Cell Entry of Porcine Epidemic Diarrhea Coronavirus Is Activated by Lysosomal Proteases. J Biol Chem, 291, 24779-24786.

  • Matsuyama, S., Ujike, M., Morikawa, S., Tashiro, M.,Taguchi, F., 2005. Protease-mediated enhancement of severe acute respiratory syndrome coronavirus infection. Proc Natl Acad Sci U S A, 102, 12543-12547.

  • Matsuyama, T., Kubli, S.P., Yoshinaga, S.K., Pfeffer, K.,Mak, T.W., 2020. An aberrant STAT pathway is central to COVID-19. Cell Death Differ, 27, 3209-3225.

  • Mazzon, M.,Marsh, M., 2019. Targeting viral entry as a strategy for broad-spectrum antivirals. F1000Res, 8.

  • Mazzon, M., Ortega-Prieto, A.M., Imrie, D., Luft, C., Hess, L., Czieso, S., Grove, J., Skelton, J.K., Farleigh, L., Bugert, J.J., Wright, E., Temperton, N., Angell, R., Oxenford, S., Jacobs, M., Ketteler, R., Dorner, M.,Marsh, M., 2019. Identification of Broad-Spectrum Antiviral Compounds by Targeting Viral Entry. Viruses, 11.

  • Mccartney, E.M., Helbig, K.J., Narayana, S.K., Eyre, N.S., Aloia, A.L.,Beard, M.R., 2013. Signal transducer and activator of transcription 3 is a proviral host factor for hepatitis C virus. Hepatology, 58, 1558-1568.

  • Medicine, U.S.N.L.O. 2022. Niclosamide clinical trials[Online]. Available:https://clinicaltrials.gov/ct2/show/NCT04542434[Accessed 20 January 2022].

  • Millet, J.K.,Whittaker, G.R., 2015. Host cell proteases:Critical determinants of coronavirus tropism and pathogenesis. Virus Res, 202, 120-134.

  • Okemoto, K., Wagner, B., Meisen, H., Haseley, A., Kaur, B.,Chiocca, E.A., 2013. STAT3 activation promotes oncolytic HSV1 replication in glioma cells. PLoS One, 8, e71932.

  • Park, J.E., Cruz, D.J.,Shin, H.J., 2014. Clathrin- and serine proteases-dependent uptake of porcine epidemic diarrhea virus into Vero cells. Virus Res, 191, 21-29.

  • Prabhakara, C., Godbole, R., Sil, P., Jahnavi, S., Zanten, T., Sheth, D., Subhash, N., Chandra, A., Nuthakki, V.K.,Puthiyapurayil, T.P.J.C.S.H.L., 2020. Niclosamide inhibits SARS-CoV2 entry by blocking internalization through pH-dependent CLIC/GEEC endocytic pathway. BioRxiv.

  • Qi, X., Cao, Y., Wu, S., Wu, Z.,Bao, W., 2021. miR-129a-3p Inhibits PEDV Replication by Targeting the EDA-Mediated NF-κB Pathway in IPEC-J2 Cells. Int J Mol Sci, 22.

  • Qin, H.R., Kim, H.J., Kim, J.Y., Hurt, E.M., Klarmann, G.J., Kawasaki, B.T., Duhagon Serrat, M.A.,Farrar, W.L., 2008. Activation of signal transducer and activator of transcription 3 through a phosphomimetic serine 727 promotes prostate tumorigenesis independent of tyrosine 705 phosphorylation. Cancer Res, 68, 7736-7741.

  • Shi, W., Fan, W., Bai, J., Tang, Y., Wang, L., Jiang, Y., Tang, L., Liu, M., Cui, W., Xu, Y.,Li, Y., 2017. TMPRSS2 and MSPL Facilitate Trypsin-Independent Porcine Epidemic Diarrhea Virus Replication in Vero Cells. Viruses, 9.

  • Singh, S., Weiss, A., Goodman, J., Fisk, M., Kulkarni, S., Lu, I., Gray, J., Smith, R., Sommer, M.,Cheriyan, J., 2022. Niclosamide-A promising treatment for COVID-19. Br J Pharmacol, 179, 3250-3267.

  • Song, D., Moon, H.,Kang, B., 2015. Porcine epidemic diarrhea:a review of current epidemiology and available vaccines. Clin Exp Vaccine Res, 4, 166-176.

  • Stevenson, G.W., Hoang, H., Schwartz, K.J., Burrough, E.R., Sun, D., Madson, D., Cooper, V.L., Pillatzki, A., Gauger, P., Schmitt, B.J., Koster, L.G., Killian, M.L.,Yoon, K.J., 2013. Emergence of Porcine epidemic diarrhea virus in the United States:clinical signs, lesions, and viral genomic sequences. J Vet Diagn Invest, 25, 649-654.

  • Wang, Y.,Stark, G.R., 2018. A new STAT3 function:pH regulation. Cell Res, 28, 1045.

  • Wang, Y.M., Lu, J.W., Lin, C.C., Chin, Y.F., Wu, T.Y., Lin, L.I., Lai, Z.Z., Kuo, S.C.,Ho, Y.J., 2016. Antiviral activities of niclosamide and nitazoxanide against chikungunya virus entry and transmission. Antiviral Res, 135, 81-90.

  • Wei, X., She, G., Wu, T., Xue, C.,Cao, Y., 2020. PEDV enters cells through clathrin-, caveolae-, and lipid raft-mediated endocytosis and traffics via the endo-/lysosome pathway. Vet Res, 51, 10.

  • Weiss, A., Touret, F., Baronti, C., Gilles, M., Hoen, B., Nougairède, A., De Lamballerie, X.,Sommer, M.O.A., 2021. Niclosamide shows strong antiviral activity in a human airway model of SARS-CoV-2 infection and a conserved potency against the Alpha (B.1.1.7), Beta (B.1.351) and Delta variant (B.1.617.2). PLoS One, 16, e0260958.

  • Wermuth, C.G., 2004. Selective optimization of side activities:another way for drug discovery. J Med Chem, 47, 1303-1314.

  • Wu, C.J., Jan, J.T., Chen, C.M., Hsieh, H.P., Hwang, D.R., Liu, H.W., Liu, C.Y., Huang, H.W., Chen, S.C., Hong, C.F., Lin, R.K., Chao, Y.S.,Hsu, J.T., 2004. Inhibition of severe acute respiratory syndrome coronavirus replication by niclosamide. Antimicrob Agents Chemother, 48, 2693-2696.

  • Xu, J., Shi, P.Y., Li, H.,Zhou, J., 2020. Broad Spectrum Antiviral Agent Niclosamide and Its Therapeutic Potential. ACS Infect Dis, 6, 909-915.

  • Yang, L., Xu, J., Guo, L., Guo, T., Zhang, L., Feng, L., Chen, H.,Wang, Y., 2018. Porcine Epidemic Diarrhea Virus-Induced Epidermal Growth Factor Receptor Activation Impairs the Antiviral Activity of Type I Interferon. J Virol, 92.