Figure 5个 Table 1个

Expression system	Genes	Notes	References
E. coli	Subfragment of GP	Subfragment of GP (GP_1, subfragment D, 471-1104 nt) was expressed at high levels and inclusion	(Das et al., 2007)
E. coli	GP_{1, 2}	GP_{1, 2} (49-1959 nt) failed to express	(Das et al., 2007)
Cell-free	GP_{1, 2}	GP_{1, 2} (49-1959 nt) -failed to express	(Das et al., 2007)
Baculovirus	GP	Full-length GP and a terminally-deleted GP (78 nt shorter than GP) was expressed	(Mellquist-Riemenschneider et al., 2003; Bengtsson et al., 2016)
	GP+VP40	Full-length GP was expressed with VP40	(Ye et al., 2006; Sun et al., 2009)
	GP+NP+VP40	Full-length GP was expressed with NP and VP40	(Warfield et al., 2007a)
Mammalian cells	Fragment of GP	Fragment of GP (residues 33-632), without the mucin-like and transmembrane domains (residues 312-462 and 633-676), was expressed	(Lee et al., 2008)
	GP	Full-length GP or GP without the mucin-like domain were expressed	(Bhattacharyya and Hope, 2011; Kuhl et al., 2011)
	GP+VP40	Full-length GP was expressed with VP40	(Noda et al., 2002; Warfield et al., 2003; Swenson et al., 2005; Melito et al., 2008)
	GP+NP	Full-length GP was expressed with NP	(Pushko et al., 2000)
	GP+NP+VP40	Full-length GP was expressed with NP and VP40	(Johnson et al., 2006; Warfield et al., 2007b; Li et al., 2016)
Notes: GP, glycoprotein; NP, nucleoprotein; VP, viral protein

Table 1. Expression of EBOV glycoprotein (GP) in different expression systems