Figure 8个  Table 1个
    • Gene Mutation Diagnostic site Wildtype Recombinant
      MVA082L (L3L) V110A ∆ Hph I (ggtga) ttg gTg aga
      lys VAL arg      		 ttg gCg aga
      lys ALA arg
      MVA113L Ava I* (ctcgag)
      Psp XI* (vc/tcgagb)      		 tgT tcT TCt
      cys ser ser      		 tgC tcG AGt
      cys ser ser
      MVA114L (A3L) Nco I* (c/catgg) tca atg gat
      ser met asp      		 tcc atg gat
      ser met asp
      H639Y aga Cat att
      arg HIS ile      		 aga Tat att
      arg TYR ile
      MVA120L (A9L) K75E aag Aag aat
      lys LYS asn      		 aag Gag aat
      lys GLU asn
      EcoR I* (g/aattc)
      ∆ Xcm I (ccan9tgg)      		 ccA aat tca ttt tgg
      pro asn ser phe trp      		 ccG aat tca ttt tgg
      pro asn ser phe trp
      MVA121L (A10L) K554K with
      Sty I (ccwwgg)      		 ccA aaG gtA
      pro lys val      		 ccC aaG gtC
      pro lys val
      MVA145R (A34R) D86Y Acc I* (gt/atac)
      ∆ BsaW I (a/ccgga)      		 aga ccg Gat act
      arg pro ASP thr      		 aga ccg Tat act
      arg pro TYR thr
      The silent mutations in MVA113L and MVA121L are markers to confirm that recombination includes the complete flanks. Note that GenBank sequence U94848 lists a mutation (cca aGA gta, R554K) in A10L at this site. However, this deviation is corrected in a subsequent analysis so that U94848 and AY603355 are considered identical (Antoine et al. 2006).

      Table 1.  Summary of mutations observed in MVA-CR19 and silent mutations used as markers in recombinant viruses.