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Group Generation of vaccinated strain Vaccination dose Generation of the challenge strain Challenge day Challenge dose 1 p95 103.5 EID50 p5 25 104.5 EID50 2 p95 104.5 EID50 p5 25 104.5 EID50 3 p105 103.5 EID50 p5 25 104.5 EID50 4 p105 104.5 EID50 p5 25 104.5 EID50 5 – – p5 25 104.5 EID50 6 – – – – – Table 1. Specific grouping for evaluation of vaccine effectiveness.
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Group Generation/Vaccination dose Morbidity Mortality 1 p5/104.5 EID50 10/10 7/10 2 p70/104.5 EID50 5/10 3/10 3 p80/104.5 EID50 3/10 3/10 4 p80/103.5 EID50 3/10 0/10 5 p95/103.5 EID50 2/10 0/10 6 p95/104.5 EID50 2/10 0/10 7 p105/103.5 EID50 2/10 0/10 8 p105/104.5 EID50 2/10 0/10 Negative control – 0/10 0/10 Table 2. The morbidity and mortality of chickens after vaccination of CK/CH/GD/GZ14 strain of different generation.
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Group Generation of vaccinated strain Vaccinated dose Generation of the challenge strain Anatomy results Morbidity (n = 10 in each group) Protection rate 1 p105 103.5 EID50 p5 1 chicken showed mild kidney enlargement and 1 chicken showed kidney enlargement 2/10 80% 2 p105 104.5 EID50 p5 1 chicken showed mild kidney enlargement, 1 chicken showed kidney enlargement and yolk malabsorption 2/10 80% 3 p95 103.5 EID50 p5 2 chickens showed kidney enlargement 2/10 80% 4 p95 104.5 EID50 p5 2 chickens showed kidney enlargement 2/10 80% 5 – – p5 10 chickens showed kidney enlargement and urate deposition 10/10 0 6 – – – The kidneys of all chickens were normal 0 – Table 3. Protective efficacy of the attenuated virus CK/CH/GD/GZ14 of different generation to 10-day-old SPF chickens against challenge with the CK/CH/GD/GZ14 virus of the 5th generation.
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Gene Nucleotide position p5–p50 p50–p80 p80–p95 p95–p105 p105–p120 Amino acid position Amino acid insertion and replacement 5'-UTR 16 – insert A – – – – – 5'-UTR 18 – A → T – – – – – 5'-UTR 21 – deletion T – – – – – 1a 2854 G → T – – – – 957 V → F 1a 3625 G → T – – – – 1209 A → S 1a 6844 – T → C – – – 2249 W → R 1a 7471 T → C – – – – 2491 Y → H 1a 8280 A → G – – – – 2707 Q → R 1a 10, 498 C → A – – – – 3500 Q → K 1a 11, 457 T → A – – – – 3820 Silent 1a 11, 786 C → T – – – – 3929 A → V 1b 1414 – G → T – – – 423 A → S E 209 – – G → T – – 70 R → I S1 353 C → T – – – – 118 P → L S1 535 – A → G – – – 179 T → A S1 1159 – G → T – – – 387 V → F S1 1232 – G → T – – – 411 R → L S2 665 – – – C → T – 222 A → V S2 1034 C → T – – – – 345 S → F S2 1403 C → T – – – – 468 S → F 5a 94 – – G → A – – 32 E → K N 434 – – C → T – – 145 P → L N 1207 G → C – – – – 403 A → P Table 4. Summary of nucleotide and amino acid mutations during CK/CH/GD/GZ14 passage.
Figure 7 个
Table 4 个