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Name Primer sequence(5′-3′) Annealing temperature (Tm, ℃) Product(bp) Primer location* JZ-P1 F ACCTGACAAAGATTTCAACTTTGGG 55 807 117–923 R GACAAGTGGGACGTTGCAAGTGTAA JZ-P2 F CCAAGAGGGGGGTGATTGGCCT 55 312 1–312 R TCCATCCTCAAGTCCACCAGTAATC JZ-P3 F GCAAATATAACCATCAGTGACAAGC 53 804 712–1515 R CATCAAGCCCTCATAGCACACATTC JZ-P4 F TTCGTTGTTATAAAACCAGGTGCGT- 54 709 1369–2077 R TTTGTAAGAATGGTGCAAGAATCCC JZ-P5 F AGAACTCGACGACTGTCGTCACACA 50 719 1919–2637 R TGCTTTAGTTAGTCGGTGTGGCGCT JZ-P6 F AGGCACCAATCTGGGAATCTTATGA 50 768 2549–3316 R GTGAATTCTCTTATGTATGGTGCCC JZ-P7 F TGGGTGGGCACCATACATAAGAGAA 50 822 3287–4108 R TTTTCTGGAGAAGTTGGCACGGGTT JZ-P8 F GTGGGTTAAGCCTGGGAAGGTCATC 53 812 4001–4812 R AGTACCATTGACAGCAGATGCGCCA JZ-P9 F GGCGTTAGGTGCACAATATTCCATG 52 665 4722–5386 R CCTTGACTAACCAGTTGAACGGCGG JZ-P10 F CAGACACAGTATGGCAGGTGCTCAA 51 728 5303–6030 R AGTGGCAGACGCCCTGTAAAAGT JZ-P11 F TATAGATTTTCAACCATCCGAGGCG 55 817 5817–6633 R ATGAGACCATGCACTGTTTGGGCTT 3'-GSP1 ATTCACCTGATAATGCCTTTGCC 54 400 6396–terminal 3'-GSP2 AGAGGACGAAGACGACGAAGCG 50 Note: *HAstV-1 Oxford L23513.1 Table Table S1. Primer sequences used for RT-PCR analysis of complete genome of HAstV-1 JZ strain
Figure 1 个
Table 1 个
