Figure 5个  Table 3
    • Phage name Isolate source Diameter#
      (cm)
      Optimal MOI Latent period*
      (min)
      Burst size*
      (ratio)
      P-PSG-1 Zhengdian, Hubei
      Province, China
      0.4 10–3 / 10.5
      P-PSG-2 Zhengdian, Hubei
      Province, China
      0.6 10–2 / 15.5
      P-PSG-3 East-Lake, Hubei
      Province, China
      0.5 10–3 20 1200
      P-PSG-4 East-Lake, Hubei
      Province, China
      0.5 10–3 20–30 65.8
      P-PSG-5 Qingshan Lake, Hubei
      Province, China
      0.5 10–4 / 2.7
      P-PSG-6 BuHe, Anhui
      Province, China
      0.1 10–4 15 191.4
      P-PSG-7 River Sare,
      Migori, Kenya
      0.5 10–5 90 200
      P-PSG-8 River Migori,
      Migori, Kenya
      0.5 10–5 50–70 53.0
      P-PSG-9 Stella Well,
      Kisumu, Kenya
      0.4 10–3 70 330
      P-PSG-10 River Rianyago,
      Migori, Kenya
      0.4 10–4 70 633.3
      P-PSG-11 River Nyambira,
      Migori, Kenya
      0.4 10–4 80 124.8
      P-PSG-12 Mwamogesha Spring,
      Migori, Kenya
      0.4 10–5 10–20 198.6
      Note: #, diameter of plaque; *, latent period and burst size are derived from adsorption kinetics curve and one-step growth curves.

      Table 1.  Partial characterization of the 12 bacteriophages

    • Group Plant no. Day of first
      observation of wilt
      after inoculation
      Day of complete
      wilt after
      inoculation
      Group Plant no. Day of first
      observation of wilt
      after inoculation
      Day of complete
      wilt after
      inoculation
      G 1 5 10 X 1 4 6
      2 5 9 2 5 7
      3 7 11 3 6 9
      4 10 15 4 7 10
      5 11 16 5 10 12
      P1-G 1 / / P1-X 1 / /
      2 / / 2 5 10
      3 / / 3 / /
      4 / / 4 / /
      5 10 18 5 / /
      G-P1-3d 1 / / X-P1-3d 1 7 13
      2 / / 2 7 14
      3 / / 3 5 10
      4 / / 4 3 6
      5 3 8 5 9 16
      G-P1-6d 1 / / X-P1-6d 1 7 12
      2 6 / 2 3 5
      3 / / 3 10 16
      4 7 / 4 5 9
      5 / / 5 3 6
      G-P1-9d 1 5 9 X-P1-9d 1 6 9
      2 7 12 2 3 6
      3 / / 3 3 6
      4 9 18 4 7 9
      5 / / 5 5 7
      G-P1-12d 1 5 9 X-P1-12d 1 3 5
      2 6 10 2 4 6
      3 4 9 3 5 7
      4 10 15 4 3 6
      5 / / 5 9 12
      G-P1-15d 1 5 9 X-P1-15d 1 5 7
      2 9 14 2 9 11
      3 4 8 3 3 5
      4 4 9 4 7 10
      5 9 14 5 3 6

      Table 2.  Disease progression in potato plants following inoculation of R. solanacearum under different bacteriophage treatments

    • Group name Titer (PFU) MOI Source
      P-PSG-1 3.06×1010 1 Single P-PSG-1
      P-PSG-2 1.84×1010 1 Single P-PSG-2
      P-PSG-3 8.0×1010 1 Single P-PSG-3
      P-PSG-4 9.8×1010 1 Single P-PSG-4
      P-PSG-5 7.2×1010 1 Single P-PSG-5
      P-PSG-6 3.0×1010 1 Single P-PSG-6
      P-PSG-7 1.0×1010 1 Single P-PSG-7
      P-PSG-8 1.8×1010 1 Single P-PSG-8
      P-PSG-9 1.3×1010 1 Single P-PSG-9
      P-PSG-10 1.42×1010 1 Single P-PSG-10
      P-PSG-11 1.4×1010 1 Single P-PSG-11
      P-PSG-12 2.42×1010 1 Single P-PSG-12
      P-PSG-(1/2) 1010 1 Equivalent volume mixture of P-PSG-1, P-PSG-2
      P-PSG-(1/8) 1010 1 Equivalent volume mixture of P-PSG-1, P-PSG-8
      P-PSG-(3/10) 1010 1 Equivalent volume mixture of P-PSG-3, P-PSG-10
      P-PSG-(6/11) 1010 1 Equivalent volume mixture of P-PSG-6, P-PSG-11
      P-PSG-(1/4/7) 1010 1 Equivalent volume mixture of P-PSG-1, P-PSG-4, P-PSG-7
      P-PSG-(1/7/12) 1010 1 Equivalent volume mixture of P-PSG-1, P-PSG-7, P-PSG-12
      P-PSG-(2/9/10) 1010 1 Equivalent volume mixture of P-PSG-2, P-PSG-9, P-PSG-10
      P-PSG-(3/5/12)10101Equivalent volume mixture of P-PSG-3, P-PSG-5, P-PSG-12
      P-PSG-(4/8/9)10101Equivalent volume mixture of P-PSG-4, P-PSG-8, P-PSG-9
      P-PSG-(5/7/9)10101Equivalent volume mixture of P-PSG-5, P-PSG-7, P-PSG-9
      P-PSG-(6/8/11)10101Equivalent volume mixture of P-PSG-6, P-PSG-8, P-PSG-11
      P-PSG-(1/2/7/12)10101Equivalent volume mixture of P-PSG-1, P-PSG-2, P-PSG-7, P-PSG-12
      P-PSG-(1/3/8/12)10101Equivalent volume mixture of P-PSG-1, P-PSG-3, P-PSG-8 P-PSG-12
      P-PSG-(2/3/4/5)10101Equivalent volume mixture of P-PSG-2, P-PSG-3, P-PSG-4, P-PSG-5
      P-PSG-(2/4/9/11)10101Equivalent volume mixture of P-PSG-2, P-PSG-4, P-PSG-9, P-PSG-11
      P-PSG-(3/5/10/12)10101Equivalent volume mixture of P-PSG-3, P-PSG-5, P-PSG-10, P-PSG-12
      P-PSG-(6/8/10/12)10101Equivalent volume mixture of P-PSG-6, P-PSG-8, P-PSG-10, P-PSG-12
      P-PSG-(1/2/3/4/6)10101Equivalent volume mixture of P-PSG-1, P-PSG-2, P-PSG-3, P-PSG-4, P-PSG-6
      P-PSG-(1/3/6/8/11)10101Equivalent volume mixture of P-PSG-1, P-PSG-3, P-PSG-6, P-PSG-8, P-PSG-11
      P-PSG-(2/3/4/5/8)10101Equivalent volume mixture of P-PSG-2, P-PSG-3, P-PSG-4, P-PSG-5, P-PSG-8
      P-PSG-(2/3/4/6/11)10101Equivalent volume mixture of P-PSG-2, P-PSG-3, P-PSG-4, P-PSG-6, P-PSG-11
      P-PSG-(2/4/5/7/9)10101Equivalent volume mixture of P-PSG-2, P-PSG-4, P-PSG-5, P-PSG-7, P-PSG-9
      P-PSG-(3/6/8/10/12)10101Equivalent volume mixture of P-PSG-3, P-PSG-6, P-PSG-8, P-PSG-10, P-PSG-12
      P-PSG-(4/6/7/9/11)10101Equivalent volume mixture of P-PSG-4, P-PSG-6, P-PSG-7, P-PSG-9, P-PSG-11
      P-PSG-(1/2/3/4/5/6)10101Equivalent volume mixture of P-PSG-1, P-PSG-2, P-PSG-3, P-PSG-4, P-PSG-5, P-PSG-6
      P-PSG-(1/2/3/7/8/9)=P110101Equivalent volume mixture of P-PSG-1, P-PSG-2, P-PSG-3, P-PSG-7, P-PSG-8, P-PSG-9
      P-PSG-(2/3/6/7/9/11)10101Equivalent volume mixture of P-PSG-2, P-PSG-3, P-PSG-6, P-PSG-7, P-PSG-9, P-PSG-11
      P-PSG-(3/4/5/8/9/10)10101Equivalent volume mixture of P-PSG-3, P-PSG-4, P-PSG-5, P-PSG-8, P-PSG-9, P-PSG-10
      P-PSG-(4/5/6/10/11/12)10101Equivalent volume mixture of P-PSG-4, P-PSG-5, P-PSG-6, P-PSG-10, P-PSG-11, P-PSG12
      P-PSG-(7/8/9/10/11/12)10101Equivalent volume mixture of P-PSG-7, P-PSG-8, P-PSG-9, P-PSG-10, P-PSG-11, P-PSG-12
      P-PSG-(1/2/3/4/6/7/12)10101Equivalent volume mixture of P-PSG-1, P-PSG-2, P-PSG-3, P-PSG-4, P-PSG-6, P-PSG-7, P-PSG-12
      P-PSG-(1/2/3/4/6/11/12)10101Equivalent volume mixture of P-PSG-1, P-PSG-2, P-PSG-3, P-PSG-4, P-PSG-6, P-PSG-11, P-PSG-12
      P-PSG-(2/3/4/5/8/9/10)10101Equivalent volume mixture of P-PSG-2, P-PSG-3, P-PSG-4, P-PSG-5, P-PSG-8, P-PSG-9, P-PSG-10
      P-PSG-(1/2/3/4/5/6/7/8/9/10/11/12)10101Equivalent volume mixture of P-PSG-1, P-PSG-2, P-PSG-3, P-PSG-4, P-PSG-5, P-PSG-6, P-PSG-7P-PSG-8, P-PSG-9, P-PSG-10, P-PSG-11, P-PSG-12
      Host broth only1010 (CFU)/No bacteriophages present
      Bacteriophage cocktail only1010/No host; equivalent volume mixture of P-PSG-1, P-PSG-2, P-PSG-3, P-PSG-4, P-PSG-5, P-PSG-6, P-PSG-7P-PSG-8, P-PSG-9, P-PSG-10, P-PSG-11, P-PSG-12

      Table S1.  Bacteriophage cocktails used in lysis kinetics analyses