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Phage name Isolate source Diameter#
(cm)Optimal MOI Latent period*
(min)Burst size*
(ratio)P-PSG-1 Zhengdian, Hubei
Province, China0.4 10–3 / 10.5 P-PSG-2 Zhengdian, Hubei
Province, China0.6 10–2 / 15.5 P-PSG-3 East-Lake, Hubei
Province, China0.5 10–3 20 1200 P-PSG-4 East-Lake, Hubei
Province, China0.5 10–3 20–30 65.8 P-PSG-5 Qingshan Lake, Hubei
Province, China0.5 10–4 / 2.7 P-PSG-6 BuHe, Anhui
Province, China0.1 10–4 15 191.4 P-PSG-7 River Sare,
Migori, Kenya0.5 10–5 90 200 P-PSG-8 River Migori,
Migori, Kenya0.5 10–5 50–70 53.0 P-PSG-9 Stella Well,
Kisumu, Kenya0.4 10–3 70 330 P-PSG-10 River Rianyago,
Migori, Kenya0.4 10–4 70 633.3 P-PSG-11 River Nyambira,
Migori, Kenya0.4 10–4 80 124.8 P-PSG-12 Mwamogesha Spring,
Migori, Kenya0.4 10–5 10–20 198.6 Note: #, diameter of plaque; *, latent period and burst size are derived from adsorption kinetics curve and one-step growth curves. Table 1. Partial characterization of the 12 bacteriophages
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Group Plant no. Day of first
observation of wilt
after inoculationDay of complete
wilt after
inoculationGroup Plant no. Day of first
observation of wilt
after inoculationDay of complete
wilt after
inoculationG 1 5 10 X 1 4 6 2 5 9 2 5 7 3 7 11 3 6 9 4 10 15 4 7 10 5 11 16 5 10 12 P1-G 1 / / P1-X 1 / / 2 / / 2 5 10 3 / / 3 / / 4 / / 4 / / 5 10 18 5 / / G-P1-3d 1 / / X-P1-3d 1 7 13 2 / / 2 7 14 3 / / 3 5 10 4 / / 4 3 6 5 3 8 5 9 16 G-P1-6d 1 / / X-P1-6d 1 7 12 2 6 / 2 3 5 3 / / 3 10 16 4 7 / 4 5 9 5 / / 5 3 6 G-P1-9d 1 5 9 X-P1-9d 1 6 9 2 7 12 2 3 6 3 / / 3 3 6 4 9 18 4 7 9 5 / / 5 5 7 G-P1-12d 1 5 9 X-P1-12d 1 3 5 2 6 10 2 4 6 3 4 9 3 5 7 4 10 15 4 3 6 5 / / 5 9 12 G-P1-15d 1 5 9 X-P1-15d 1 5 7 2 9 14 2 9 11 3 4 8 3 3 5 4 4 9 4 7 10 5 9 14 5 3 6 Table 2. Disease progression in potato plants following inoculation of R. solanacearum under different bacteriophage treatments
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Group name Titer (PFU) MOI Source P-PSG-1 3.06×1010 1 Single P-PSG-1 P-PSG-2 1.84×1010 1 Single P-PSG-2 P-PSG-3 8.0×1010 1 Single P-PSG-3 P-PSG-4 9.8×1010 1 Single P-PSG-4 P-PSG-5 7.2×1010 1 Single P-PSG-5 P-PSG-6 3.0×1010 1 Single P-PSG-6 P-PSG-7 1.0×1010 1 Single P-PSG-7 P-PSG-8 1.8×1010 1 Single P-PSG-8 P-PSG-9 1.3×1010 1 Single P-PSG-9 P-PSG-10 1.42×1010 1 Single P-PSG-10 P-PSG-11 1.4×1010 1 Single P-PSG-11 P-PSG-12 2.42×1010 1 Single P-PSG-12 P-PSG-(1/2) 1010 1 Equivalent volume mixture of P-PSG-1, P-PSG-2 P-PSG-(1/8) 1010 1 Equivalent volume mixture of P-PSG-1, P-PSG-8 P-PSG-(3/10) 1010 1 Equivalent volume mixture of P-PSG-3, P-PSG-10 P-PSG-(6/11) 1010 1 Equivalent volume mixture of P-PSG-6, P-PSG-11 P-PSG-(1/4/7) 1010 1 Equivalent volume mixture of P-PSG-1, P-PSG-4, P-PSG-7 P-PSG-(1/7/12) 1010 1 Equivalent volume mixture of P-PSG-1, P-PSG-7, P-PSG-12 P-PSG-(2/9/10) 1010 1 Equivalent volume mixture of P-PSG-2, P-PSG-9, P-PSG-10 P-PSG-(3/5/12) 1010 1 Equivalent volume mixture of P-PSG-3, P-PSG-5, P-PSG-12 P-PSG-(4/8/9) 1010 1 Equivalent volume mixture of P-PSG-4, P-PSG-8, P-PSG-9 P-PSG-(5/7/9) 1010 1 Equivalent volume mixture of P-PSG-5, P-PSG-7, P-PSG-9 P-PSG-(6/8/11) 1010 1 Equivalent volume mixture of P-PSG-6, P-PSG-8, P-PSG-11 P-PSG-(1/2/7/12) 1010 1 Equivalent volume mixture of P-PSG-1, P-PSG-2, P-PSG-7, P-PSG-12 P-PSG-(1/3/8/12) 1010 1 Equivalent volume mixture of P-PSG-1, P-PSG-3, P-PSG-8 P-PSG-12 P-PSG-(2/3/4/5) 1010 1 Equivalent volume mixture of P-PSG-2, P-PSG-3, P-PSG-4, P-PSG-5 P-PSG-(2/4/9/11) 1010 1 Equivalent volume mixture of P-PSG-2, P-PSG-4, P-PSG-9, P-PSG-11 P-PSG-(3/5/10/12) 1010 1 Equivalent volume mixture of P-PSG-3, P-PSG-5, P-PSG-10, P-PSG-12 P-PSG-(6/8/10/12) 1010 1 Equivalent volume mixture of P-PSG-6, P-PSG-8, P-PSG-10, P-PSG-12 P-PSG-(1/2/3/4/6) 1010 1 Equivalent volume mixture of P-PSG-1, P-PSG-2, P-PSG-3, P-PSG-4, P-PSG-6 P-PSG-(1/3/6/8/11) 1010 1 Equivalent volume mixture of P-PSG-1, P-PSG-3, P-PSG-6, P-PSG-8, P-PSG-11 P-PSG-(2/3/4/5/8) 1010 1 Equivalent volume mixture of P-PSG-2, P-PSG-3, P-PSG-4, P-PSG-5, P-PSG-8 P-PSG-(2/3/4/6/11) 1010 1 Equivalent volume mixture of P-PSG-2, P-PSG-3, P-PSG-4, P-PSG-6, P-PSG-11 P-PSG-(2/4/5/7/9) 1010 1 Equivalent volume mixture of P-PSG-2, P-PSG-4, P-PSG-5, P-PSG-7, P-PSG-9 P-PSG-(3/6/8/10/12) 1010 1 Equivalent volume mixture of P-PSG-3, P-PSG-6, P-PSG-8, P-PSG-10, P-PSG-12 P-PSG-(4/6/7/9/11) 1010 1 Equivalent volume mixture of P-PSG-4, P-PSG-6, P-PSG-7, P-PSG-9, P-PSG-11 P-PSG-(1/2/3/4/5/6) 1010 1 Equivalent volume mixture of P-PSG-1, P-PSG-2, P-PSG-3, P-PSG-4, P-PSG-5, P-PSG-6 P-PSG-(1/2/3/7/8/9)=P1 1010 1 Equivalent volume mixture of P-PSG-1, P-PSG-2, P-PSG-3, P-PSG-7, P-PSG-8, P-PSG-9 P-PSG-(2/3/6/7/9/11) 1010 1 Equivalent volume mixture of P-PSG-2, P-PSG-3, P-PSG-6, P-PSG-7, P-PSG-9, P-PSG-11 P-PSG-(3/4/5/8/9/10) 1010 1 Equivalent volume mixture of P-PSG-3, P-PSG-4, P-PSG-5, P-PSG-8, P-PSG-9, P-PSG-10 P-PSG-(4/5/6/10/11/12) 1010 1 Equivalent volume mixture of P-PSG-4, P-PSG-5, P-PSG-6, P-PSG-10, P-PSG-11, P-PSG12 P-PSG-(7/8/9/10/11/12) 1010 1 Equivalent volume mixture of P-PSG-7, P-PSG-8, P-PSG-9, P-PSG-10, P-PSG-11, P-PSG-12 P-PSG-(1/2/3/4/6/7/12) 1010 1 Equivalent volume mixture of P-PSG-1, P-PSG-2, P-PSG-3, P-PSG-4, P-PSG-6, P-PSG-7, P-PSG-12 P-PSG-(1/2/3/4/6/11/12) 1010 1 Equivalent volume mixture of P-PSG-1, P-PSG-2, P-PSG-3, P-PSG-4, P-PSG-6, P-PSG-11, P-PSG-12 P-PSG-(2/3/4/5/8/9/10) 1010 1 Equivalent volume mixture of P-PSG-2, P-PSG-3, P-PSG-4, P-PSG-5, P-PSG-8, P-PSG-9, P-PSG-10 P-PSG-(1/2/3/4/5/6/7/8/9/10/11/12) 1010 1 Equivalent volume mixture of P-PSG-1, P-PSG-2, P-PSG-3, P-PSG-4, P-PSG-5, P-PSG-6, P-PSG-7P-PSG-8, P-PSG-9, P-PSG-10, P-PSG-11, P-PSG-12 Host broth only 1010 (CFU) / No bacteriophages present Bacteriophage cocktail only 1010 / No host; equivalent volume mixture of P-PSG-1, P-PSG-2, P-PSG-3, P-PSG-4, P-PSG-5, P-PSG-6, P-PSG-7P-PSG-8, P-PSG-9, P-PSG-10, P-PSG-11, P-PSG-12 Table S1. Bacteriophage cocktails used in lysis kinetics analyses
Figure 5 个
Table 3 个