Figure 2个  Table 2个
    • Dilution TCID50/mL Average Ctvalue±standard deviationa
      10−1 2.32 × 103 21.34±0.07
      10−2 2.32 × 102 24.53±0.10
      10−3 2.32 × 101 28.12±0.39
      10−4 2.32 × 100 31.38±0.17
      10−5 2.32 × 10−1 39.51±0.21
      10−6 2.32 × 10−2 No Ct
      10−7 2.32 × 10−3 No Ct
      10−8 2.32 × 10−4 No Ct
      Negative controlb - No Ct
      Note:a: The Ct values were calculated based on the dRn threshold of 0.27 adapted from (Ben Shabat et al., 2010); b: DEPC treated water.

      Table 1.  Detection limits of viral load for a Eurasian-lineage H10 specific quantitative RT-PCR method

    • Host Virus name
      Human A/Puerto Rico/8/1934(H1N1)
      A/California/04/2009(H1N1)
      A/Brisbane/10/2005(H3N2)
      A/Perth/16/2009(H3N2)
      Avian A/mallard/Oregon/A00544081/2008(H1N1)
      A/mallard/Wisconsin/A00751454/2009(H1N1)
      A/mallard/Oregon/A00300758/2008(H2N3)
      A/mallard/Oregon/A00320372/2007(H2N3)
      A/mallard/Wisconsin/A00661712/2009(H3N2)
      A/mallard/Wisconsin/A00714818/2009(H3N2)
      A/mallard/Colorado/A00170379/2006(H3N8)
      A/mallard/Wisconsin/A00170366/2006(H3N8)
      A/mallard/Washington/A00714770/2009(H4N6)
      A/mallard/Washington/A00757090/2009(H4N6)
      A/chicken/Jiangxi/JXA132321/2013(H5N?)a
      A/mallard/Colorado/A00551322/2007(H6N1)
      A/mallard/New York/A00603000/2009(H6N1)
      A/blue-winged teal/Illinois/10OS3610/2010(H6N2)
      A/mallard/Ohio/81/1986(H6N2)
      A/mute swan/Rhode Island/A00325114/2008(H7N3)
      A/american green-winged teal/Utah/A00461135/2009(H7N1)
      A/northern shoveler/Mississippi/A00630207/2009(H7N6)
      A/chicken/Jiangxi/JXA132304/2013(H9N2)a
      A/mallard/South Dakota/A00536114/2007(H10N7)
      A/mallard/New York/A00919062/2009(H10N7)
      A/chicken/Jiangxi/JXA132717/2013 (H10N8)a
      Avian Newcastle disease virus (strain 2013 pt#3-3)
      Infectious bursal disease virus (strain P13-03308)
      Infectious bronchitis virus (strain P14-00957ct)
      Reovirus (strain P14-00932)
      Note:a: The RNA from these viruses was extracted directly from a clinical swab sample; for all others viruses, the RNA was extracted from virus isolates.

      Table S1.  Viruses used to determine the specificity of a Eurasian-lineage H10 specific quantitative RT-PCR method