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Nucleotides (ANN) Amino acid Number of clones AUG* M204 WT 3 AUA* M204I 4 AUU 1 AUC 2 ACA M204T 0 ACU 2 ACC* 7 AAA M204K 5 AAG* 2 AAU* M204N 3 AAC 4 AGA M204R 3 AGG* 1 AGC M204S 0 AGU 0 37 Note: The genomic DNAs were purified from 40 clones, 37 of which were found to have the integrated polymerase gene. The number of different mutants is listed. * Cell lines with these mutants were subjected to the phenotype assay. Table 1. Characterization of monoclonal cells
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Primer 5′–3′ lenti6744F AGGTTTCCCGACTGGAAAGC Rpol204 TTGGCCCCCAATACCACATCATCNNTATAACTGAAAGCCAAACAGTGGG GGA F260 TAGACTCGTGGTGGACTTCTCT RlentiPCR GAATCGAGACCGAGG AGAGGGT F1927 GGAGCTACTGTGGAGTTACTCT R2051 TTGCCTGAGTGCAGTATGGTGA Table S1. Primer sequences
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Nucleotides (ANN) Amino acid Number of clones AUG M204 WT 3 AUA M204I 1 AUU M204I 1 AUC M204I 2 ACA M204T 5 ACU M204T 1 ACC M204T 2 ACG M204T 0 AAA M204K 3 AAG M204K 1 AAU M204N 1 AAC M204N 2 AGA M204R 4 AGG M204R 1 AGC M204S 2 AGU M204S 1 Total 30 Table S2. Distribution of mutations among the 30 plasmids sequenced
Figure 1 个
Table 3 个
