Figure 5个  Table 1个
    • Gene Sequences (5′-3′) a Amplicon size (bp) Annealing (℃) Accession no.
      IBV-N F:CAGAAGAAGGGCTCTGCATTAC
      R:AGGTTGAGCATTGCCGTAACAC      		 200 60 FJ548847.1
      TLR3 F:GCAACACTTCATTGAATAGCCTTGAT
      R:GCCAAACAGATTTCCAATTGCATGT      		 92 62.5 EF137861.1
      TLR7 F:AAGTCCCGGTATGTTCAGCT
      R:GGACAGGGTATTGTTCATAGC      		 132 58.1 NM_0001011688.2
      MyD88 F:CAGCGAGCAATAGCATCCAG
      R:CAACAGAGCCACCTTGTATC      		 104 61 NM_001030962.1
      TRIF F:TTCAGCCATTCTCCGTCCTC
      R:GCCAATGATGCTTCCACAG      		 120 60 NM_001081506.1
      MDA5 F:CAGCCAGTTGCCCTCGCCTCA
      R:AACAGCTCCCTTGCACCGTCT      		 210 60 NM_001193638
      LGP2 F:CCAGAATGAGCAGCAGGAC
      R:AATGTTGCACTCAGGGATGT      		 109 60 XM_004948615
      MAVS F:CCTGACTCAAACAAGGGAAG
      R:AATCAGAGCGATGCCAACAG      		 123 60 NM_001012893
      STING F:TGACCGAGAGCTCCAAGAAG
      R:CGTGGCAGAACTACTTTCAG      		 82 60 XM_001232170
      TBK1 F:AAGAAGGCACACATCCGAGA
      R:GGTAGCGTGCAAATACAGC      		 170 58.1 NM_001199558.1
      IKKε F:TGGATGGGATGGTGTCTGAAC
      R:TGCGGAACTGCTTGTAGATG      		 117 60 XM_428036.4
      TRAF3 F:GGACGCACTTGTCGCTGTTT
      R:CGGACCCTGATCCATTAGCAT      		 107 60 XM_004936348.1
      TRAF6 F:GATGGAGACGCAAAACACTCAC
      R:GCATCACAACAGGTCTCTCTTC      		 170 60 XM_004941547.1
      IKKα F:GAGGGGTGGAGGCTTAGATC
      R:ACTTTCCTCGGGATGCAAGA      		 141 60 NM_001012904.1
      IKKβ F:TACAGGCAATCCAGACCTTCG
      R:GACTGCCACTAACAGGACCAC      		 234 60 NM_001031397.1
      NF-κB p65 F:CATTGCCAGCATGGCTACTAT
      R:TTCCAGTTCCCGTTTCTTCAC      		 102 60 NM_205129.1
      IRF7 F:ACACTCCCACAGACAGTACTGA
      R:TGTGTGTGCCCACAGGGTTG      		 134 58.5 NM_205372.1
      IFN-α F:GGACATGGCTCCCACACTAC
      R:TCCAGGATGGTGTCGTTGAAG      		 75 61.3 X92476.1
      IFN-β F:TTCTCCTGCAACCATCTTC
      R:GAGGTGGAGCCGTATTCT      		 82 62.5 AY974089.1
      IL-1β F:AACCCGACCAGGTCAACA
      R:CGGTACATACGAGATGGAAAC      		 101 61.3 AJ245728.1
      IL-6 F:ATCCCTCCTCGCCAATCTG
      R:CCTCACGGTCTTCTCCATA      		 103 61.3 HM179640.1
      IL-8 F:CACAGCTCCACAAAACCTCA
      R:GTCCTACCTTGCGACAGAGC      		 118 60 NM_205498
      IL-10 F:ATGCTGCGCTTCTACACA
      R:CCATGCTCTGCTGATGACT      		 73 61.3 AY647438.1
      IL-12 F:TCTGCTAAGACCCACGAGA
      R:TTGACCGTATCATTTGCCCAT      		 82 60 NM_213571
      MIP-1β F:TGCTGGTGTTGTGTTCATC
      R:GTCCTGTACCCAGTCGTT      		 73 63 L34553.1
      GAPDH F:GGTGGTGCTAAGCGTGTTA
      R:CCCTCCACAATGCCAA      		 179 60.5 X01578.1
      TBP F:TAGCCCGATGATGCCGTAT
      R:TTCCCTGTGTCGCTTGC      		 147 62.5 D83127
      Note: a Primers for TLR3, IFN-α were previously reported (Karpala et al., 2008), and those for GAPDH and TBP were also previously reported (Li et al., 2005). F indicates forward primer; R indicates reverse primer.

      Table .  Primers used in this study.