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Putative target mRNA Accession Number Position of binding site Nudix (nucleoside diphosphate linked moiety X)-type motif 5 (NUDT5) NM_014142.2 CDS Geminin, DNA replication inhibitor (GMNN) NM_001251989.1 CDS Ribosomal protein L5 (RPL5) NM_000969.3 CDS Polycomb group ring finger 2 (PCGF2) NM_007144.2 3′ UTR Serine/threonine/tyrosine interacting-like 1 (STYXL1) NM_016086.2 3′ UTR Deleted in liver cancer 1 (DLC1) NM_182643.2 3′ UTR SIN3 transcription regulator family member B (SIN3B) NM_015260.2 3′ UTR Ribosomal protein S18 (RPS18) NM_022551.2 3′ UTR Ornithine decarboxylase antizyme 1 (OAZ1) NM_004152.2 3′ UTR Nuclear factor I/X (CCAAT-binding transcription factor) (NFIX) NM_002501.3 3′ UTR Apoptotic peptidase activating factor 1 (APAF1) NM_013229.2 5′ UTR ATPase, H+ transporting, lysosomal 14kDa, V1 subunit F (ATP6V1F) NM_001198909.1 3′ UTR Transmembrane protein 47(TMEM47) NM_031442.3 3′ UTR Transmembrane protein 109 (TMEM109) NM_024092.2 3′ UTR Inner membrane protein, mitochondrial (IMMT) NM_006839.2 3′ UTR Ferritin, heavy polypeptide 1 (FTH1) NM_002032.2 CDS Tripartite motif containing 26 (TRIM26) NM_003449.4 3′ UTR Ral guanine nucleotide dissociation stimulator-like 1 (RGL1) NM_015149.3 3′ UTR Zinc finger, SWIM-type containing 7 (ZSWIM7) NM_001042697.1 3′ UTR Ribosomal protein L41 (RPL41) NM_021104.1 3′ UTR RAD51 paralog D (RAD51D) NM_002878.3 3′ UTR KIAA1462 NM_020848.2 3′ UTR Chromosome 20 open reading frame 194 (C20orf194) NM_001009984.2 3′ UTR Table 1. Putative targets of hcmv-miR-US5-1 identified by hybrid-PCR
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Table S1. Primer sequences used in plasmid construction
Figure 6 个
Table 2 个