Figure 4个  Table 3个
    • Flavivirus species Reference Lineage GenBank no. Virus titer (TCID50/mL) RT-qPCR assays
      This publication
      ZIKV MR_766 African KX377335 2.1 × 106 14.75 ± 0.22
      ZIKV PRVABC59 Asian KX377337 3.2 × 104 20.22 ± 0.16
      ZIKV PLCal_ZV Asian KF993678 3.2 × 105 17.59 ± 0.22
      ZIKV SZ_SMGC-1 Asian KX266255 3.2 × 105 17.96 ± 0.44
      ZIKV CAS01 Asian NA 2.7 × 105 18.59 ± 0.23
      DENV CN/GZ27/2014 Serotype 1 KP723473 NAc Ud
      DENV DENV2-43 Serotype 2 AF204178 NA U
      DENV YN02 Serotype 3 KF824903 NA U
      DENV GZB5 Serotype 4 AF289029 NA U
      YFV BJ01/2016 —b KY495641 NA U
      WNVa NY99 —b NC_009942 1 × 106(copies/μL) NA
      Note: ZIKV, Zika virus; DENV, dengue virus; YFV, yellow fever virus; WNV, West Nile virus. a The synthetic envelope gene was used instead of the live virus; b Not applicable; c Not available; d Undetected. The Ct values of the commercial kits for DENVs (CN/GZ27/2014, DENV2-43, YN02, and GZB5) and YFV were 17.22, 12.18, 15.23, 16.42, and 15.59, respectively.

      Table 1.  ZIKV and other flavivirus strains used in the present study

    • Serial dilutiona Ct Values
      MR_766 CAS01 PLCal_ZV SZ_SMGC-1 PRVABC59
      Viral stock 17.92 ± 0.04 18.69 ± 0.23 17.71 ± 0.40 18.16 ± 0.39 20.32 ± 0.06
      10–1 21.13 ± 0.18 21.80 ± 0.25 20.95 ± 0.01 21.36 ± 0.42 23.62 ± 0.18
      10–2 24.49 ± 0.06 25.15 ± 0.05 24.19 ± 0.13 24.62 ± 0.48 26.96 ± 0.02
      10–3 27.71 ± 0.11 28.51 ± 0.09 27.80 ± 0.10 27.86 ± 0.45 30.26 ± 0.04
      10–4 31.01 ± 0.18 32.25 ± 0.10 31.21 ± 0.05 31.51 ± 0.61 34.06 ± 0.10
      10–5 34.52 ± 0.01 35.89 ± 0.58 34.64 ± 0.10 34.86 ± 0.36 37.03 ± 0.31
      10–6 37.75 ± 0.22 38.66 ± 0.54 38.74 ± 0.25 37.48 ± 0.13 Neg
      10–7 Negb Neg Neg Neg Neg
      Note: aThe titers of stock ZIKV were 2.1 × 105 TCID50/mL (MR_766), 2.7 × 105 TCID50/mL (CAS01), 3.2 × 105 TCID50/mL (SZ_SMGC-1), 3.2 × 105 TCID50/mL (PLCal_ZV) and 3.2 × 104 TCID50/mL (PRVABC59), respectively; b Negative.

      Table 2.  Sensitivity of the qRT-PCR assay for ZIKV RNA detection

    • Samples qRT-PCR assays Pa
      Our method Commercial kit
      Patient 1 Serum 29.67 ± 0.82 30.63 ± 0.13 0.1146
      Urine 29.54 ± 0.61 30.47 ± 0.12 0.0606
      Patient 2 Serum 26.71 ± 0.58 27.66 ± 0.84 0.1823
      Note: aData were analyzed using the Student’s t-test, and P-values represent a comparison of our method with that of the commercial kit.

      Table 3.  Detection of ZIKV in clinical samples