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Family Morphology Phage species Host References Myoviridae Contractile tail AS-1 Aancystis nidulans, Synechococcus cedrorum Safferman R S, et al., 1972 N-1 Nostoc muscorum Adolph K W, et al., 1971 Ma-LMM01 Microcystis aeruginosa Yoshida T, et al., 2006 Long, non-contractile tail SM-2 Synechococcus elongates, Microcystis aeruginosa Fox J A, et al., 1976 Siphoviridae S-2L Synechococcus sp. 698 Khudyakov I Y, et al., 1978 S-4L Synechococcus. elongatus Khudyakov I Y, et al., 1982 LPP-1 Lyngbya, Plectonema, Phormidium Sherman L A, et al, 1970 Podoviridae Short tail SM-1 Synechococcus elongatus Safferman R S, et al., 1969 Ma-LBP Microcystis aeruginosa Tucker S, et al., 2005 Unassigned Tailless PaV-LD Planktothrix agardhii Gao E B, et al., 2009 Table 1. Examples of freshwater cyanophages
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Name Family Host Genome structure Genome
size (bp)No. of
ORFs% G+C Coding %
of genomeGenBank
Accession numberMa-LMM01 Myoviridae Microcystis Circularly permuted linear 162109 184 45.0 92.0 AB231700 S-CRM01 Myoviridae Synechococcus Circularly permuted linear 178563 294 39.7 89.0 HQ615693 Pf-WMP4 Podoviridae Phormidium Linear 40938 41 51.8 89.9 DQ875742 Pf-WMP3 Podoviridae Phormidium Linear 43249 41 46.5 89.2 EF537008 PP-1 Podoviridae Plectonema Linear 42480 41 46.4 93.1 Unpublished PaV-LD unassigned Planktothrix Linear 95299 142 41.5 89.5 HQ683709 Table 2. Freshwater cyanophages with completely sequenced genomes
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Family Host Gene Gene product Primer sequences (5'-3') References Myoviridae Microcystis g91 Sheath protein Sheath RTF: ACATCAGCCTTCGTTTCGG Takashima Y, et al., 2007 Sheath RTR: CAATCTGGTTAGGTAGGTCG Anabaena and Nostoc MCP Major capsid protein AN15 MCPF5: GTTCCTGGCACACCTGAAGCGT Baker A C, et al., 2006 AN15 MCPR5: CTTACCATCGCTTGTGTCGGCATC Uncertain g20 Viral capsid assembly protein CPS1: GTAGWATTTTCTACATTGAYGTTGG Wilhelm S W, et al., 2006 CPS8: AAATAYTTDCCAACAWATGGA Synechococcus g20 Viral capsid assembly protein CPS4: CATWTCWTCCCAHTCTTC Short C M, et al., 2005 G20-2: SWRAAATAYTTICCRACRWAKGGATC The codes for mixed bases are: R = A, G; Y = C, T; M = A, C; K = G, T; S = C, G; W = A, T; H = A, C, T; B = C, G, T; V = A, C, G; D = A, G, T; n = A, G, C, T; I = inosine. Table 3. Diagnostic markers for freshwater cyanophages
Figure 1 个
Table 3 个
