Figure 2个  Table 5个
    • Norovirus GI, DNA copies/mL Assay Aa Assay Ba Assay Ca
      Mean Cq ± SDb Mean Cq ± SDb Mean Cq ± SDb
      1 × 106 25.26 ± 0.23 25.72 ± 0.36 23.85 ± 0.86
      1 × 105 29.58 ± 0.54 28.75 ± 0.16 25.63 ± 0.01
      1 × 104 34.20 ± 0.39 31.77 ± 0.36 –
      1 × 103 36.11 ± 0.48 35.06 ± 0.74 ND
      1 × 102 – – ND
      Notes: –, negative; ND, not done. aAssay A: Norovirus Real Time RT-PCR kit; Assay B: LightCycler RNA Master HybProbe; Assay C: RealTime ready RNA Virus Master; b3–4 repeat experiments.

      Table 1.  The analytical sensitivities of three real-time RT-PCR assays for the detection of norovirus GI

    • Norovirus GII, DNA copies/mL Assay Aa Assay Ba Assay Ca
      Mean Cq ± SDb Mean Cq ± SDb Mean Cq ± SDb
      1 × 105 30.22 ± 0.93 27.09 ± 1.50 31.15 ± 1.89
      1 × 104 34.24 ± 1.26 29.08 ± 0.78 32.99 ± 1.11
      1 × 103 36.13 ± 0.87 31.89 ± 1.08 34.64 ± 0.89
      1 × 102 – – –
      Notes: –, negative. aAssay A: Norovirus Real Time RT-PCR kit; Assay B: LightCycler RNA Master HybProbe; Assay C: RealTime ready RNA Virus Master; b3–4 repeat experiments.

      Table 2.  The analytical sensitivities of three real-time RT-PCR assays for the detection of norovirus GII

    • Assaya No. of positive samples/total (%) Quantification cycle (Cq) Noroviurs GI, DNA copies/mL
      Mean Range Mean Range
      A 5/18 (27.8) 29.58 22.17–31.88 2.00 × 106 1.03 × 104–9.33 × 106
      B 3/18 (16.7) 28.44 26.92–29.70 4.08 × 105 1.93 × 104–1.17 × 106
      C 1/18 (5.6) 21.55 21.55 1.85 × 106 1.85 × 106
      P-value 0.050b –c –c
      Notes: a: Assay A: Norovirus Real Time RT-PCR kit; Assay B: LightCycler RNA Master HybProbe method; Assay C: RealTime ready RNA Virus Master method; b: Cochran's Q test; c: Statistical analysis was not determined because Cq and DNA copies/mL of Method C were obtained from one norovirus GI-positive sample (i.e. there is no mean Cq or DNA copies/mL value).

      Table 3.  The detection and quantification of norovirus GI in fecal samples using three real-time RT-PCR assays

    • Assaya No. of positive samples/total (%) Quantification cycle (Cq) Noroviurs GII, DNA copies/mL
      Mean Range Mean Range
      A 38/43 (88.4) 28.38 13.04–35.36 7.27 × 107 4.56 × 102–2.11 × 109
      B 39/43 (90.7) 25.79 15.67–30.20 4.83× 107 7.29 × 102–1.24 × 109
      C 35/43 (81.4) 28.06 13.77–34.25 1.21× 108 2.20 × 102–3.47 × 109
      P-value 0.368b 0.000c 0.846c
      Notes: a: Assay A: Norovirus Real Time RT-PCR kit; Assay B: LightCycler RNA Master HybProbe method; Assay C: RealTime ready RNA Virus Master method; b: Cochran's Q test; c: Friedman test.

      Table 4.  The detection and quantification of norovirus GII in fecal samples using three real-time RT-PCR assays

    • Norovirus genotype No. of samples Assay Aa Assay Ba Assay Ca
      GI.2 1 1 1 1
      GI.6 2 2 – –
      GII.2 2 1 1 1
      GII.3 3 2 2 2
      GII.4 2006a 2 2 2 2
      GII.4 2006b 13 11 12 10
      GII.6 2 2 2 2
      GII.12 1 1 1 1
      GII.17 1 1 1 1
      GII.21 7 6 7 6
      Total (%) 34 29 (85.3) 29 (85.3) 26 (76.5)
      Notes: a: Assay A: Norovirus Real Time RT-PCR kit; Assay B: LightCycler RNA Master HybProbe; Assay C: RealTime ready RNA Virus Master.

      Table 5.  Norovirus genotypes detected by three real-time RT-PCR assays