Figure 6个  Table 2个
    • GradeClinical sign (s)
      0 Healthy
      1 Lethargy and listlessness
      2 Emaciation
      3 Limb weakness
      4 Hair thinning, hunching or weakness in ≥ two limbs
      5 Moribund and death
      CVB1 Coxsackievirus B1.

      Table 1.  Grading score for the clinical symptoms of CVB1-infected mice.

    • ExperimentNumber of miceOnset of symptoms (d) Onset of death (d) The time of all mice died (d) Mortality ratio (%)
      1 7 3 4 10 100
      2 6 3 4 10 100
      3 6 3 4 8 100
      4 8 4 5 11 100
      5 6 3 4 9 100
      The results of five replicate and independent experiments that were challenged by a CVB1 clinical stain (10 TCID50 per mouse) in neonatal BALB/c mice at different times are shown. The clinical symptoms and mortality were monitored and recorded daily, which showed good reproducibility.
      CVB1 Coxsackievirus B1; TCID50 median tissue culture infective dose.

      Table 2.  The repeatability and stability of the CVB1-challenged neonatal mouse model.