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Tissues* MFI Brain* 1698 Heart* 1621.5 Liver* 1613.5 Spleen* 848.5 Lung* 1261.5 Kidney* 1692 Brain from the healthy mouse* 3 CCHFV 1369 Blank 38 Note: *Tissues were collected from newborn mice sacrificed on 5 d p.i. MFI, median fluorescence intensity of each sample. Table 1. MFI values of the tissues from newborn mice
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Primers Sequences (5'-3') Locations of primers S segment S1F* TCTCAAAGAAACACGTGCCGC 1-21 S1R* GACAAATTCCCTGCACCA 653-670 S2F* CCCAGTGAGCCGTGAACAT 628-646 S2R* CAGGGTGCATGTAGATCCTG 1168-1187 S3F* CTTTATGAGCTCTTTGCTGATG 1130-1151 S3R* TCTCAAAGATATCGTTGCCGC 1652-1672 M segment M1F* TCTCAAAGAAATACTTGCGGCAC 1-22 M1R* ATGRTGTGYAYYYTGTGGTGT 504-524 M2F* GTGCATGCAAGCCCATCACC 504-523 M2R* CRTTGATCARCTGMARYCCTG 1201-1221 M3 F TTACTATGCCAAAGGGTCTC 1151-1170 M3 R ATTTGACTGGGATGCTATCT 1901-1920 M4F* TWYAAGCTDTGCGAGAACAGTGC 1833-1855 M4R* CTNACAACCCARGGDATTCTTTC 2481-2503 M5F* CGGARGGARAARGTRGAAGAAACY 2439-2462 M5R* GTWGGTTTGAAGGTTGAYTGRACAT 3307-3331 M6F-2‡* CGTGGGGAGCAATCAATGTTC 3316-3336 M6R-2‡* GTGTGCAGCTCTGTAGCTTGC 4030-4050 M7F* GAAGGTTTYTTTGAYYTRATGCATG 3951-3975 M7R* GCTCTGTGGWTGSTCAAGYTT 4719-4739 M8F* AGCTGCASYRAAGAWGAYACACAA 4680-4703 M8R* TCTCAAAGATATAGTGGCGGCAC 5345-5367 L segment L1 F TCTCAAAGATATCAATCCCC 1-20 L1 R GAGTGCTAATGCAAGGTCTT 652-671 L2F* CTCTCAGAATACTGCCACAG 538-557 L2R* GTTGATAACATGACGCCAAGTG 1123-1144 L3F* CCCTGGAACAGGAATAGAAAG 1052-1072 L3R* ATTGCCCTGCCTGAGGTAC 1666-1684 L4-F1 ACTTCTCGGCTTATCACATT 1500-1519 L4-R1 ATTCAACGGAGTCTCAGGTA 2287-2306 L5 F CAAACCAAGTCTGACCATGT 2048-2067 L5 R TCTGTCCTTATTTTCCCGTC 2571-2590 L6F* GACTATGGAGAGAGGGGAATAG 2463-2484 L6R* TGACTTGATGTCTTCACTTAACC 3130-3152 L7 F CTACCCGTTAGTTTGGAACA 2934-2953 L7 R CTATCTTGGGACACCTCTTG 3311-3330 L8 F GATTGAGCTGCTTGCTTATA 3218-3237 L8 R GACCTTGGCACAGAACACTT 3723-3742 L9 F CAACCTATGCAAAAGGAGTA 3661-3680 L9 R TATGATGAGCCAAGGACAGC 4287-4306 L10+ F AGTAAAGGTAAGGCATTGTG 4154-4173 L10+ R GTTTATTCTGTTGTTGGGAG 4616-4635 L11+ F ACCTAGTTTATGGCTTCCTT 4573-4592 L11+ R ACTGGCAGATGTCTGTTATT 5309-5328 L10F* TGCCCAAATGTGAGAAAAGC 5076-5095 L10R* GAGYYTCTTCTTYAAGCTTCC 5889-5909 L11F* CAATCTCTTCHTCAGTYAAAGG 5869-5890 L11R* CTATCTTTGTYTGATGTAGYAGC 6614-6636 L12F* GAYYTRCACAARACCACTGACG 6588-6609 L12R* CTGACCCATATGGTTGTARCTGTT 7473-7496 L13 F TAGACAGTTGGGAAGGAAAT 7396-7415 L13 R TAAAGGCAGTGTTGGTGATA 7955-7974 L14 F CTTATCACCAACACTGCCTTTA 7953-7974 L14 R AAACTCCCAACCTTTCCAAC 8699-8718 L15 F CATTTGGAGGAGAAGGAACT 8587-8606 L15 R AAGGCTCTTTGACAGTGGTAT 9106-9126 L16+ F GATGGTCCACCAAGCACAGC 8980-8999 L16+ R TGCCCTTTAGCATGTAGAAA 9656-9675 L17 F TACAACAAGCAAAGCCAACC 9628-9647 L17 R GTATGTGAACTTCCCAGACC 10129-10148 L16F* CTGTRAAACGAGATTCTGAACGC 10000-10022 L16R* ACCTCCTGATTTGAGGTACTTGTG 10794-10817 L18+ F AGACTACATACCACGAGAATC 10564-10584 L18+ R CTCTGGAAATAGAAGGCACA 11277-11296 L19 F GTTAATGAACTTAATCAGCCAGTG 11183-11206 L19 R ACCCGTCAACATCAGAATCAAATC 11718-11741 L18F* CAAAGCCTGARAGAGTGGTYATRG 11473-11496 L18R* TCTCAAAGAAATCGTTCCCCCCAC 12133-12156 Note: *Primers were used in reference to a previous study of CCHFV genome announcement (Zhou et al., 2013b). Table S1. Primers used to sequence the complete genome of the CCHFV isolate
Figure 4 个
Table 2 个
