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Name Sequence (5′-3′)a RE site Pacvp39-UP CGATTACGTACTCAAGTTGTGCGAAAC SnaB Ⅰ Pacvp39-DP CTCGGATCCAATATTGTTGCCGTTATAAAT BamH Ⅰ Ppxvp39-UP TGCATACGTAGGATCCCCGTAAAC SnaB Ⅰ Ppxvp39-DP GGCGGGATCCAATCATTTTAATGTTTTAATT BamH Ⅰ Ppxvp39L-UP TCATCGTGTGCGGTAAAAAGGGTTC - Ppxvp39-DP CCGGAATTCTCGCAGGCTAATAATTTTAATGTTT EcoR Ⅰ Pacp6.9-UP GGTCGACGTACCAAATTCCGTTTTGCGACG - Pacp6.9-DP GGTCGACGGATCCGTTTAAATTGTGTAATTTATG BamH Ⅰ Ppxp6.9-UP CCCCTCGTTATCGAAAACATTGCCAT - Ppxp6.9-DP ATAGGATCCGGCGTCTTACCAGTTTGTCT BamH Ⅰ Pace25-UP CTAAGGCCTTGTTCGATGCAATGAT Stu Ⅰ Pace25-DP CCCCACAGGATCCGTTTTAATTTAC BamH Ⅰ Ppxe25-UP CTGAATACGGTAGCTCGTCGGCG - Ppxe25-DP CGCGGATCCCAACAGTTTTTATTCTC BamH Ⅰ Pace18-UP CGCGCCCAAGCAGCGTATATTAAGT - Pace18-DP CGCGGATCCATTATTGTACCGAGTC BamH Ⅰ Ppxe18-UP CTCAAACCGGGAGACGTCCTGTACC - Ppxe18-DP CGCGCGGATCCCTTATTATTTTATCTTATG BamH Ⅰ Pacgp41-UP GCATTACGTATCGGCGCGTCAGTTTT SnaB Ⅰ Pacgp41-DP CGGGCATCTGGGATCCTTTTTATTTGT BamH Ⅰ Ppxgp41-UP TTGCGTGCTGCTAACCGTATTGAT - Ppxgp41-DP TCTGGATCCTAGTCTTAAAGATAACGGC BamH Ⅰ Ppxgp41L-UP GAGCTGACCGAATACGTAGAGGGAG SnaB Ⅰ Ppxgp41-DP TCTGGATCCTAGTCTTAAAGATAACGGC BamH Ⅰ Pacpk1-UP TCGTCGACGTACGTATATGCTTTGTTGT SnaB Ⅰ Pacpk1-DP TGGCGGATCCGAATCGTAGATATGAAT BamH Ⅰ Ppxpk1-UP AATCGACGTACGAGTGCGTGGAG - Ppxpk1-DP GCGTGGATCCAAACGATAACAATGT BamH Ⅰ Ppxgran-UP GCCCAACATACCGGACGCTATCACAAC - Ppxgran-DP CGCGGATCCAATGTTTTTGTAAAAATAAAAATTC BamH Ⅰ Ppxorf21-UP GGCGTTTCACGTAAAATTGTGCTCAT - Ppxorf21-DP TACGGATCCTGGAGATGTTATCGAAATT BamH Ⅰ Ppxorf50-UP TTTACGTACTGCGGAAACGGTTGC - Ppxorf50-DP GGCGGGATCCGTAAATCTTCTTATGTAT BamH Ⅰ Ppxe18n95x-U GATCGACAAGTAATGTCAAGGGTGTAAAAAAATT - Ppxe18n95x-D GACATTACTTGTCGATCGCACAATTATGTACAT - Ppxe18n76x-U GGGTGTAAAAAAATTACGTCAATTAAAAAAC - Ppxe18n76x-D GTAATTTTTTTACACCCTTAACATTACTTG - Ppxe18n20x-U TAAATTTATTTGTAACATACGATAAAATAATAAG - Ppxe18n20x-D GTATGTTACAAATAAATTTAAAAGCGTATTC - Ppxe18n7x-UP CATAAGATAAAATAATACGGGATCCCGGTC - Ppxe18n7x-DP GTATTATTTTATCTTATGTTACAAATAAATTT - Note: a sequences of restriction sites are underlined; the mutated late promoter motifs of PlxyGV e18 promoter are in bold and underlined. Table S1. PCR primers used in this study
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Table 1 个
