Figure 1个  Table 3个
    • Sow no. Foetus no.* PCV2 strain PCV2 replication
      Heart Lungs Spleen Liver Kidneys Thymus Tonsils Ileum Cerebrum
      VT IIF VT IIF VT IIF VT IIF VT IIF VT IIF VT IIF VT IIF VT IIF
      S1 L1 K2 6.3 900 3.0 253 5.7 NA 5.2 10353 2.7 587 5.6 NA 3.7 NA 4.1 NA 2.0 10
      S2 R3 K2 5.5 976 4.7 2363 6.3 23803 5.3 12110 5.0 2890 5.8 7073 4.8 3416 4.3 3850 2.5 13
      S3 L2 K2 4.0 580 3.5 1260 5.6 8320 5.0 2337 2.5 880 4.8 10383 4.1 1297 4.8 670 2.5 17
      S1 L2 K39 6.0 3550 2.5 223 4.5 1160 2.8 1337 < 1.7 10 3.0 11883 3.8 13 1.8 63 < 1.7 20
      S2 L2 K39 6.0 4027 2.2 3 3.5 80 3.9 1297 2.1 27 3.0 30 2.8 37 3.0 10 < 1.7 0
      S3 L3 K39 5.8 4350 3.0 20 3.8 163 4.3 1137 2.7 3 4.3 NA 2.8 NA 1.8 NA 2.5 0
        * L = left horn; R = right horn. Numbering of foetuses is in sequence from ovary to cervix.
        VT = Virus titres (log10 TCID50/g); IIF = Indirect immunofluorescence staining (number of PCV2-positive cells /10 mm2 ); NA = not available

      Table 1.  Virus replication in different foetal organs after intra-foetal inoculation with K2 or K39 at 55 days of gestation, collected at 21 days post-inoculation

    • Sow no. Numbering of pigs at birth Intra-foetal inoculation with Dose
      (log10 TCID50)       		Clinical outcome at birth (69 dpi)
      S4 F1 K2 4.3 live
      F2 K2 4.3 mummified
      F3 K2 4.3 mummified
      F4 mock - live
      F5 mock - live
      F6 mock - live
      S5 F1 K2 4.3 mummified
      F2 K2 4.3 mummified
      F3 K2 4.3 mummified
      F4 mock - live
      F5 mock - live
      F6 mock - live
      S6* F1 K2 2.3 live
      F2 K2 2.3 mummified
      F3 K2 2.3 mummified
      F4 mock - live
      F5 mock - live
      F6 mock - live
      S7* F1 K2 2.3 mummified
      F2 K2 2.3 mummified
      F3 K2 2.3 mummified
      F4 mock - live
      F5 mock - live
      F6 mock - live
        * live-born pigs from sows S6 and S7 were immunostimulated.

      Table 2.  Clinical outcome of pigs inoculated intra-foetally with PCV2-K2 or mock-inoculated at 45 days of gestation and collected at 69 days post-inoculation (at birth)

    • Pig no. Prenatal life Prenatal life
      Intra-uterine inoculation At 45 days of gestation Inoculated dose of K2 Oronasal inoculation at 6 or 8 days after birth PCV2 replication IPMA antibody titres
      14 dpsi 21 dpsi 28 dpsi at birth 0 dpsi 7 dpsi 14 dpsi 21 dpsi 28 dpsi
      ILN ILN ILN
      VT IIF VT IIF VT IIF K2 K39 K2 K39 K2 K39 K2 K39 K2 K39 K2 K39
      S4F1 K2 104.3 K39 < 1.7 2 2.3 0 2.3 0 < 10 < 10 < 10 < 10 < 10 < 10 < 10 < 10 10 160 160 40
      S4F4 mock K39 < 1.7 0 < 1.7 0 3.3 0 < 10 < 10 < 10 < 10 < 10 < 10 < 10 < 10 160 640 160 10240
      S4F5 mock K39 < 1.7 0 2.3 0 2.3 0 < 10 < 10 < 10 < 10 < 10 < 10 < 10 < 10 640 2560 640 10240
      S4F6 mock K39 < 1.7 0 2.3 0 2.3 0 < 10 < 10 < 10 < 10 < 10 < 10 < 10 < 10 160 2560 640 10240
      S5F4 mock mock < 1.7 0 < 1.7 0 < 1.7 0 < 10 < 10 < 10 < 10 < 10 < 10 < 10 < 10 < 10 < 10 < 10 < 10
      S5F5 mock mock < 1.7 0 < 1.7 0 < 1.7 0 < 10 < 10 < 10 < 10 < 10 < 10 < 10 < 10 < 10 < 10 < 10 < 10
      S5F6 mock mock < 1.7 0 < 1.7 0 < 1.7 0 < 10 < 10 < 10 < 10 < 10 < 10 < 10 < 10 < 10 < 10 < 10 < 10
      S6F1 K2 102.3 K39 5.6 76 5.1 86 1.9 8 < 10 < 10 640 640 640 640 640 640 10240 2560 40960 10240
      S6F4 mock K39 < 1.7 0 3.0 2 2.3 0 < 10 < 10 < 10 < 10 < 10 < 10 < 10 < 10 160 2560 640 10240
      S6F5 mock K39 < 1.7 0 2.0 0 < 1.7 0 < 10 < 10 < 10 < 10 < 10 < 10 40 40 640 2560 2560 40960
      S6F6 mock K39 < 1.7 0 2.7 0 < 1.7 0 < 10 < 10 < 10 < 10 < 10 < 10 < 10 < 10 160 2560 2560 10240
      S7F4 mock mock < 1.7 0 < 1.7 0 < 1.7 0 < 10 < 10 < 10 < 10 < 10 < 10 < 10 < 10 < 10 < 10 < 10 < 10
      S7F5 mock mock < 1.7 0 < 1.7 0 < 1.7 0 < 10 < 10 < 10 < 10 < 10 < 10 < 10 < 10 < 10 < 10 < 10 < 10
      S7F6 mock mock < 1.7 0 < 1.7 0 < 1.7 0 < 10 < 10 < 10 < 10 < 10 < 10 < 10 < 10 < 10 < 10 < 10 < 10
        IPMA = Immunoperoxidase monolayer assay. PCV2-specifi c antibody titres in pig serum during postnatal life were determined using IPMA; dpsi = days post super-inoculation; ILN = inguinal lymph node; VT = Virus titres (log10 TCID50/g); IIF = Indirect immunofl uorescence staining (number of PCV2-positive cells/10 mm2 ); Immunostimulated pigs are shown in bold.

      Table 3.  PCV2 replication and IPMA antibody titres in pigs inoculated with PCV2-K2 (or mock-inoculated) during prenatal life (at 45 days of gestation) followed by oronasal inoculation with PCV2-K39 (or mock-inoculation) during postnatal life