Figure 6个  Table 3个
    • Isolate name GenBank no Country Genotype Mismatches
      cr1a cr2b cr3c
      E75 FN557520.1 Spain Ⅰ 0 0 0
      Georgia 2007/1 NC_044959.1 Georgia Ⅱ 0 0 0
      Wuhan 2019–1 MN393476.1 China Ⅱ 0 0 0
      Krasnodar 2012 KJ195685.1 Russia Ⅱ 0 0 0
      Pig/HLJ/2018 MK333180.1 China Ⅱ 0 0 0
      Warmbaths AY261365.1 South Africa Ⅰ/Ⅲ 0 0 0
      Warthog AY261366.1 Namibia Ⅳ 0 0 0
      Tengani 62 AY261364.1 Malawi Ⅴ 0 0 0
      Mkuzi 1979 AY261362.1 South Africa Ⅰ/Ⅶ 0 0 2
      Ken06.Bus NC_044946.1 Kenya Ⅸ 2 0 2
      Ken05/Tk1 KM111294.1 Kenya Ⅹ 2 0 2
      Pretoriuskop/96/4 AY261363.1 South Africa ⅩⅩ/Ⅰ 0 0 0
      a, b, c crRNA-1, crRNA-2 and crRNA-3, respectively

      Table 1.  Analysis of mismatches on crRNA targeted regions of P72 genes from different genotype strains.

    • Plasmid concentration (copies/μL) Ct value sample 1 Ct value sample 2 Ct value sample 3 Standard deviation(SD) Variation coefficient(CV)
      5 × 107 12.11 12.20 12.21 0.055 0.45%
      5 × 106 15.36 15.40 15.46 0.050 0.32%
      5 × 105 18.57 18.57 18.65 0.046 0.24%

      Table 2.  Intra-assay variation coefficient of qPCR.

    • Plasmid concentration (copies/μL) Ct value sample 1 Ct value sample 2 Ct value sample 3 Standard deviation(SD) Variation coefficient(CV)
      5 × 107 12.09 12.47 12.75 0.331 2.66%
      5 × 106 15.36 15.58 16.26 0.469 2.98%
      5 × 105 18.73 19.21 19.26 0.296 1.53%

      Table 3.  Inter-assay variation coefficient of qPCR.