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Viruses LD50×103 OBs (95% CI) Potency ratioa (95% CI) vHaBac-egfp-ph 9.2 (6.7, 12.5) - vHaBac-Δvfgf-egfp-ph 16.9 (12.4, 22.7) 1.8* (1.2, 2.9) vHaBac-REPvfgf-egfp-ph 8.7 (6.4, 12.0) 0.9 (0.6, 1.5) Note: a Potency ratio was calculated by dividing the LD50 of the vfgf-deleted or -repaired variants by that of vHaBac-egfp-ph. * Indicates significant difference based on the 95% CI of the potency ratio, including the value 1.0 (Robertson et al., 2007). CI, confidence interval. Table 1. Dose-mortality responses of vHaBac-egfp-ph, vHaBac-Δvfgf-egfp-ph, and vHaBac-REPvfgf-egfp-ph in third-instar H. armigera larvae
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Tests Viruses ST50 (95% CI) (h) χ2 P 1 vHaBac-egfp-ph 95 (93.2, 96.7) - - vHaBac-Δvfgf-egfp-ph 107.5 (103.1, 111.9) 27.834 1×10-6 * vHaBac-REPvfgf-egfp-ph 94.5 (93.1, 95.9) 3.568 0.059 2 vHaBac-egfp-ph 95 (93.1, 96.9) - - vHaBac-Δvfgf-egfp-ph 107.5 (104.5, 110.5) 19.915 8×10-6* vHaBac-REPvfgf-egfp-ph 94.5 (93.6, 95.4) 3.327 0.068 Note: * Indicates significant difference between the vfgf-deleted variant and vHaBac-egfp-ph based on log-rank test. CI, confidence interval. Table 2. Time-mortality responses of vHaBac-egfp-ph, vHaBac-Δvfgf-egfp-ph, and vHaBac-REPvfgf-egfp-ph in third-instar H. armigera larvae.
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Primer Primer sequence (5′-3′) anti-vFGF-f GCGGGATCCAGACCGGGCGGACGAAAC anti-vFGF-r GCGAAGCTTCATGTACGTTACAACAACAG vFGF trans-f CGTGCCGTTATATTTGCGTTAACCAATGC vFGF trans-r CAGAGGTAACATGGATGATGTCTGTGG del-F1f CGGGGTACCATACAAATTCAACCTGCGAACTC del-F1r CCGCTCGAGATCATCCATGTTACCTCTGAC del-F2f CCGGATATCGTGCGAGTCAGTAGAATTTGTT del-F2r CTAGTCTAGAATTTCTAACACATTCTATGCGATTG vfgf-f GCGCTCGAGTGGAGCAGATTCAAGATTCGCC vfgf-r GCGGGTACCCATGTACGTTACAACAACAG Table S1. Sequences of primers used for PCR amplification
Figure 3 个
Table 3 个