-
-
-
-
-
-
-
-
Fragementsa Primersb, c, d P74△3TM F CGCGAATTCTTCATGTCGAATATCATTTATATAC R GCGAAGCTTTTATTTAATTGCTATTTTAGTCATC N F GCCGGATTCTTCATGTCGAATACATTTATATACA R CGGCTCGGGCAATAGGCTTCGTTGAAAAAAA M F CGCGGATCCCTAGGCGATACTGTACTTGTC R CCCAAGCTTGCGCGTTGCCATCTATCCGTTG C F CGCGGATCCCGTTCCTTACAACCGAACGG R CCCAAGCTTGCCGCCGTAACGGTTTTAATAGC Ⅰ F CGCGGATCCTTCATGTCGAATATCATTTATATAC R CCCAAGCTTGCGTAAAAATCATCATTGT TAGCCG Ⅱ F CGCGGATCCTTCATACCGAAATGGCGGG R CCCAAGCTTGCCGATGTTTGTGTATACCCGAA Ⅲ F CGCGGATCCGAAAGCATGAGTTGTTATCCG R CCCAAGCTTGCTGTCAAAGTGTCCACTATAATAC Ⅳ F CGCGGATCCGGTGCCGAAAATGAAATACAAA R CCCAAGCTTGCGCAATAGGCTTCGTTGAAAAAA Ⅲ F1 F CGCGGATCCCAGTTCGGGTATACACAAACATCGGAAACGGCAGTGGCGTACGCTCAACCCGCATTCATGTCGAATATCATTTATATAC Ⅲ F2 F CGCGGATCCGCAGTGGCGTACGCTCAACCCGCATGCTACAATTTGGACAGGGCGGCGGCGGTGTTCATGTCGAATATCATTTATATAC Ⅲ F3 F CGCGGATCCAATTTGGACAGGGCGGCGGCGGTGCGCGACGGTGCCGAAAATGAAATACAAACGTTCATGTCGAATATCATTTATATAC Ⅳ F1 F CGCGGATCCAAATGTATTATAGTGGACACTTTGACAAAAATGTATTTGAACACTCCCTATTTGCGTACCCAACCTACTGTACCTACGCC Ⅳ F2 F CGCGGATCCTTGAACACTCCCTATTTGCGTACCGATGACCATTTGATACAGGGCGTTGATGATGTGCCCCAACCTACTGTACCTACGCC Ⅳ F3 F CGCGGATCCATACAGGGCGTTGATGATGTGCCCGGATTCAATGTGACAAACGATACGGATCAACTTTTTCAACCTACTGTACCTACGCC Ⅳ F4 F CGCGGATCCACAAACGATACGGATCAACTTTTTCCCGAAAGATTCGAAGGTTTTTTCAACGAAGCCTATTGCCAACCTACTGTACCTACGCC Ⅲ F3(1) F CGCGGATCCAATTTGGACAGGGCGGCGGCGGTGCGCTTCATGTCGAATATCATTTATATAC Ⅲ F3(2) F CGCGGATCCGACAGGGCGGCGGCGGTGCGCGACGGTTCATGTCGAATATCATTTATATAC Ⅲ F3(3) F CGCGGATCCGCGGCGGCGGTGCGCGACGGTGCCGAATTCATGTCGAATATCATTTATATAC Ⅲ F3(4) F CGCGGATCCGCGGTGCGCGACGGTGCCGAAAATGAATTCATGTCGAATATCATTTATATAC Ⅲ F3(5) F CGCGGATCCCGCGACGGTGCCGAAAATGAAATACAAACGTTCATGTCGAATATCATTTATATAC Ⅳ F4(1) F CGCGGATCCACAAACGATACGGATCAACTTTTTCCCTTCATGTCGAATATCATTTATATAC Ⅳ F4(2) F CGCGGATCCGATACGGATCAACTTTTTCCCGAAAGATTCATGTCGAATATCATTTATATAC Ⅳ F4(3) F CGCGGATCCGATCAACTTTTTCCCGAAAGATTCGAATTCATGTCGAATATCATTTATATAC Ⅳ F4(4) F CGCGGATCCCTTTTTCCCGAAAGATTCGAAGGTTTTTTCATGTCGAATATCATTTATATAC Ⅳ F4(5) F CGCGGATCCCCCGAAAGATTCGAAGGTTTTTTCAACTTCATGTCGAATATCATTTATATAC Ⅳ F4(6) F CGCGGATCCAGATTCGAAGGTTTTTTCAACGAAGCCTTCATGTCGAATATCATTTATATAC Ⅳ F4(7) F CGCGGATCCGAAGGTTTTTTCAACGAAGCTATTGCTTCATGTCGAATATCATTTATATAC a The reverse primer used for the amplification of fragments Ⅲ F1 - Ⅳ F4(7) was the same as that used for the amplification of fragment Ⅰ; b Primer sequences are 5′ to 3′; c Restriction enzyme sites are underlined; d F indicates forward primer; R indicates reverse primer. Table 1. Primers for the PCR amplification of truncated and chimeric P74 gene fragments
Figure 7 个
Table 1 个
