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Detection systems Target Detection sensitivity (copies/µL) Assay reaction time (approximate) Assay components Bulky instrumentation required Reference OneStep RT-PCR assay N gene 1 90 min Denature (50 ℃, 15 min; 94 ℃, 15 min) amplification (94 ℃, 15 s; 55 ℃, 45 s; 45 cycles) Yes This study RT–LAMP ORF1ab gene 10 (ORF1ab) 60 min RT–LAMP (63 ℃ for 60 min) No Yan et al. (2020) S gene 100 (S) ORF1ab gene 0.08 30 min RT–LAMP (65 ℃ for 30 min) No Huang et al. (2020) S gene N gene RT–LAMP/Cas12 E gene 10 30–40 min RT–LAMP (62 ℃, 20–30 min) No Broughton et al. (2020) N gene Cas12 (37 ℃, 10 min) Cas13-based
SHERLOCK systemS gene 46.2 (S gene highest sensitivity) 60 min RT–RPA (42 ℃, 25 min) No Patchsung et al. (2020) ORF1ab gene Cas13 (37 ℃, 30–60 min) N gene RT-ERA N gene 10 80 min RT–ERA (39 ℃, 30 min) No This study Purification (56 ℃, 5 min) Agarose gel electrophoresis (45 min) Cas12a-based
RT-ERA systemN gene 0.25 (N) 40 min RT–ERA (39 ℃, 30 min) No This study ORF1ab gene 0.5 (ORF1ab) Cas12a (37 ℃, 10 min) Table 1. Comparison of detection methods for SARS-CoV-2
Figure 2 个
Table 1 个