Figure 5个  Table 4个
    • Time Sampling location Number of groups Total ticks
      2015 NJ 76 912
      LX 45 540
      2016 DL 351 4212
      LX 47 564
      2017 LX 79 410
      Total 574 7387

      Table 1.  Summary of total tick sample collections in Yunnan Province from 2015 to 2017

    • No. Virus name Closest relative Identity with relative virus (aa) Classification of relative virus Coverage (%) RPKM
      1 Yunnan mivirus 1 (YNMV1) strain YNMV1 Wuhan mivirus strain X78-1 86% Mivirus 94.2 4.8
      2 Wuhan tick vrius 1 (WHTV1) strain YNrhabdoV1 Wuhan tick vrius 1 strain X78-2 97% Unassigned Rhabdovirdae 83.3 2.5
      3 YN tick-associated phlebovirus 1 (YNPhelobV1) strain YN-PLV1 Lihan tick virus strain LH-1 S segment 89%
      L segment 88%      		 Phlebovirus 86 1.57
      1.9
      4 Jingmen tick virus (JMTV) strain YNflaviV Jingmen tick virus isolate SY84 S1 segment 93%
      S2 segment 99%
      S3 segment 96%
      S4 segment 99%      		 Unssigned (Jingmenvirus) S1:87.7
      S2:84.5
      S3:86
      S4:87.2      		 1.33
      2.7
      1.8
      3.0
      5 Bovine hokovirus 2 (BHKV) strain YNBHKV2 Bovine hokovirus 2 strain HK-B38 100% Tetraparvovirus 97 1.30
      6 Bole mivirus (BLMV) strain YN-MV2 Bole tick virus 3 strain BL199 95% Mivirus 44.1 0.09
      7 Bole tick virus 1 (BLTV1) strain YNPLV2 Bole tick virus 1 strain BL075 S/L segment 98% Phlebovirus 36.4 0.05
      8 YN tick-associated flavi-like virus 1 (YN-FlaviV1) strain YN-FLV 1 Bole tick virus 4 80% Unassigned (Flavi-like) 30.2 0.03
      9 YN tick-associated phlebovirus (YNPhelobV1) strain YN-PLV3 Blacklegged tick virus S segment 60%
      L segment 65%      		 Phlebovirus 23.2 0.01
      0.01
      RPKM: Reads Per Kilobase per Million mapped reads

      Table 2.  Viruses identified in R. microplus in our study

    • 2015 2016 2017
      NJ
      (76)      		 LX
      (45)      		 DL
      (353)      		 LX
      (41)      		 LX
      (79)
      Yunnan mivirus 1 28.9%
      (22/76)      		 24.4%
      (11/45)      		 24.9%
      (88/353)      		 19.5%
      (8/41)      		 17.8%
      (14/79)
      Wuhan tick vrius 1 42%
      (32/76)      		 12.9%
      (4/45)      		 15.8%
      (56/353)      		 4.8%
      (2/41)      		 5%
      (4/79)
      YN tick-associated phlebovirus 1 2.6%
      (3/76)      		 11.1%
      (5/45)      		 18.4%
      (7/31)      		 4.8%
      (2/41)      		 15%
      (12/79)
      Jingmen tick virus 0 1/45
      (2.2%)      		 0 0 0
      Bovine hokovirus 2 0 0 0 0 63.3%
      (50/79)

      Table 3.  PCR-based epidemiological survey of each virus among tick samples

    • Cattle Serum sample Human serum sample
      Hubei
      (n = 100)      		 Yunnan
      (n = 100)      		 Febrile patient
      (n = 100)      		 Healthy human
      (n = 100)
      Yunnan mivirus 1 0 89% (89/100) 0 0
      Wuhan tick vrius 1 0 94% (94/100) 0 0
      YN tick-associated phlebovirus 1 0 4% (4/100) 0 0
      Jingmen tick virus 0 18% (18/100) 0 0
      Bovine hokovirus 2 0 40% (40/100) 0 0

      Table 4.  IFA-based epidemiological survey targeting NP protein of each virus