Figure 4个  Table 3个
    • Segment bp ORF Position Direction Encoded protein Function
      L 6368 1 17-6271 5′-3′ RdRp Replication, transcription, and viral cap-dependent transcription.
      M 3378 2 19-1704 5′-3′ Gn Virus assembly, formation of virus particles, immunogenicity, neutralizing or protective epitopes, viral infectivity, precursor of glycoprotein.
      1705-3240 5′-3′ Gc
      S 1744 2 29-910 5′-3′ NSs Host innate immune responses, formation of viral inclusion bodies, formation of viroplasm-like structures, virus replication, transcription, replication, virion assembly, formation of RNP, viral RNA encapsidation.
      1702-965 3′ -5′ NP
      Note: *With references to: Liu et al., 2014; Lei et al., 2015; Li et al., 2011

      Table 1.  Genomic characteristics of SFTSV*

    • Symptoms Patients with symptoms (N=68)
      No. (%)
      Fever 68 (100.00)
      Malaise 49 (72.06)
      Fatigue 48 (70.59)
      Anorexia 39 (57.35)
      Nausea 21 (30.88)
      Diarrhea 19 (27.94)
      Vomiting 17 (25.00)
      Dizziness 16 (23.53)
      Headache 13 (19.12)
      Throat congestion 13 (19.12)
      Cough 12 (17.65)
      Myalgia 8 (11.76)
      Chill 7 (10.29)
      Lymphadenopathy 7 (10.29)

      Table 2.  Clinical symptoms of patients with laboratory confirmed disease in Hubei Province, 2011

    • Category Detection methods Minimum detection limit Sensitivity Specificity
      SFTSV S segment qRT-PCR (TaqMan) 10 copies/μL 98.6-100% 99-100%
      SFTSV L segment qRT-PCR (TaqMan) 5 × 10 copies/μL NA NA
      SFTSV M segment qRT-PCR (TaqMan) 2 × 102 copies/mL NA NA
      SFTSV M segment RT-LAMP 10 copies/test NA 100%
      SFTSV M segment RT-CPA-VF 100 copies/test 94.1% 100%
      SFTSV S segment qRT-PCR (AllGlo) 1 × 103 copies/mL NA NA
      SFTSV S & L segment Nest RT-PCR 10 copies/μL NA 55%
      SFTSV antibody & RNA ELISA+Nest RT-PCR NA NA 86%
      SFTSV antibody IFA NA 95.6% 74.29%
      SFTSV IgG MLBI NA 90.7% 66.67-100%
      SFTSV total antibody ELISA NA 100% 99.57-100%
      SFTSV RNA Luminex 102 copies/μL NA NA
      SFTSV IgM ELISA NA 90.59-96.67% 59-100%
      SFTSV IgM ICA NA 98.4-100% NA
      SFTSV antigen ELISA 0.117 mg NA NA
      SFTSV RNA qRT-PCR (MGB) 10 copies/μL 100% 99%
      SFTSV IgG ICA NA 96.7-98.6% NA
      Note: NA, not applicable; ICA, immunochromatographic assay; RT-LAMP, reverse transcription-loop-mediated isothermal amplification; RT-CPA, reverse transcription-cross-priming amplification; MLBI, multiplexed luminex-based immunoassay; VF, vertical flow; MGB, minor-groove-binding (Yu et al., 2015; Wang et al., 2014; Cui et al., 2012; Wu et al., 2014; Li et al., 2013; Wen et al., 2011).

      Table 3.  Comparison of various detection methods for SFTSV