Figure 1个  Table 1个
    • Facility (Patients capacity) Sampling site Concentration of chlorine residual (mg/L) Before concentration After concentration (250:1)
      qPCR result (Ct value) qPCR result (Ct value) ddPCR result (copies/reaction)
      ORF1ab N ORF1ab N ORF1ab N E
      Designated hospital
      1 Wuchang Hospital (504) Outlet of the West Zone onsite LWTS > 10 Negative Negative NT NT NT NT NT
      2 The Central Hospital of Wuhan (543) Outlet of the onsite LWTS 6 Negative Negative 35.90 33.63 134 42 10.2
      Fangcang shelter hospital
      3 Jiangxia Cabin Hospital (564) Outlet of the onsite LWTS 1 Negative Negative NT NT NT NT NT
      4 Jiangan Cabin Hospital (1000) Outlet of the onsite LWTS 3 Negative Negative 33.64 32.31 402 26 36
      Community quarantine spot
      5 Guanggu New Beacon Hotel (60) Outlet of the onsite LWTS 5 Negative Negative NT NT NT NT NT
      6 Jinyinhu New Beacon Hotel (167) Outlet of the onsite LWTS NT (high turbidity) Negative 38.96 Negative Negative 44 6.6 11
      WWTP
      7 Qingshan WWTP South 3# wastewater inlet < 0.5 Negative Negative NT NT NT NT NT
      8 Qingshan WWTP South 3# water outlet < 0.5 Negative Negative NT NT NT NT NT
      9 Hanxi WWTP Main wastewater inlet < 0.5 Negative Negative Negative 36.15 110 0 0
      10 Hanxi WWTP Main water outlet < 0.5 Negative Negative NT NT NT NT NT
      WWTP wastewater treatment plant, qPCR quantitative PCR, ddPCR droplet digital PCR, LWTS liquid waste treatment system, Ct cycle of threshold, NT not tested.

      Table 1.  Presence of SARS-CoV-2 RNA in the wastewater from different locations in Wuhan, China, during the COVID-19 outbreak