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Serum collection time rAd3H immunization HAdV-3 wildtype GZ01 inoculation Intranasal Intramuscular Intranasal Intramuscular D21 1:250 1:270 1:1000 1:1428 D28 1:200 1:250 1:1111 1:1000 D35 1:167 1:333 1:1111 1:1667 D42 1:158 1:248 1:916 1:1024 The mice were either immunized with the rAd3H recombinant vaccine or inoculated with HAdV-3 wildtype strain GZ01 intranasally or intramuscularly. At day 21, 28, 35, and 42 after the prime inoculation/immunization, the neutralizing antibodies against HAdV-3 were titrated. Table 1. Determination of the 50% neutralizing antibody titer in mouse sera by the microculture neutralization test.
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Table 1 个