Figure 3个  Table 3个
    • qRT-PCR I-RPA Total
      Positive Negative
      Positive 39 3 42
      Negative 0 53 53
      Total 39 56 95
      The results were analyzed by Kappa consistency test. Kappa = 0.936.

      Table 1.  Detected results of clinical samples by I-RPA and qRT-PCR assay.

    • Primer name Sequence (5'to 3')a Position (size, bp)b
      Cov-RPA-F2 ACATAGAAGTTATTTGACTCCTGGTGATTC 22, 269–22, 298 (30)
      Cov-RPA-F3 TAGAAGTTATTTGACTCCTGGTGATTCTTC 22, 272–22, 301 (30)
      Cov-RPA-F1 TATTTGACTCCTGGTGATTCTTCTTCAGGT 22, 279–22, 308 (30)
      Cov-RPA-P AAAGTCCTAGGTTGAAGATAACCCACATAAFAAHCQGCAGCACCAGCTGTCCA-[3'-block] 22, 309–22, 361 (53)
      Cov-RPA-R1 ACACTTTGTTTCTGAGAGAGGGTCAAGTGC 22, 411–22, 440 (30)
      CoV-RPA-R2 TCAAGTGCACAGTCTACAGCATCTGTAATG 22, 389–22, 418 (30)
      CoV-RPA-R3 AACAATAGATTCTGTTGGTTGGACTCTAAA 22, 489–22, 518 (30)
      a F: FAM-dT, thymidine nucleotide carrying fluorescein; H: THF, tetrahydrofuran spacer; Q: BHQ1-dT, thymidine nucleotide carrying Black-Hole Quencher 1; 3'-block: 3'-phosphate introduced to block elongation.
      b Numbers in position column are primer positions according to SARS-CoV-2, hCoV-19/Wuhan/WH01/2019|EPI_ISL_406798|2019-12-26

      Table Table S1.  Sequences of the primers and probe used for the RPA assay

    • Sample Results Sample Results
      I-RPA Real-time Ct value (Mean ±2SD) I-RPA Real-time Ct value (Mean ±2SD)
      P1 P 27.31±0.92 P22 P 19.8±0.18
      P2 P 26.98±0.33 P23 P 25.31±0.06
      P3 P 29.72±0.72 P24 P 22.9±0.08
      P4 P 29.81±0.07 P25 P 26.59±0.72
      P5 P 29.2±0.43 P26 P 22.88±0.32
      P6 P 29.07±0.78 P27 P 31.72±0.16
      P7 P 28.53±0.22 P28 P 30.65±0.21
      P8 P 27.66±0.56 P29 P 33.79±0.37
      P9 P 31.71±0.20 P30 P 29.46±0.52
      P10 P 32.9±0.14 P31 P 25.27±0.68
      P11 P 29.92±0.77 P32 P 35.83±0.77
      P12 P 29.2±0.65 P33 P 25.75±0.89
      P13 P 29.07±0.32 P34 P 36.58±0.48
      P14 P 31.59±0.35 P35 P 20.71±0.86
      P15 P 33.48±0.12 P36 P 35.31±0.22
      P16 P 33.14±0.42 P37 P 36±0.72
      P17 N 36.84±0.68 P38 N 38.69±0.33
      P18 N 37.11±0.32 S1 P 33.21±0.71
      P19 P 25±1.14 S2 P 29.09±1.32
      P20 P 27.88±0.98 S3 P 29.91±0.62
      P21 P 17.31±1.08 S4 P 32.83±0.88
      P: positive; N: negative; P1-P38: Pharyngeal swab samples; S1-S4:Sputum samples.
      SD: standard deviation

      Table Table S2.  Analysis of clinical samples by I-RPA and rRT-PCR