Figure 6个  Table 2个
    • PrimerSequence
      9470R5' CTATGGAGTGTACCTAGTGTGTGC 3'
      S175' GGCGACACTCCGCCATGAATC 3'
      R52855' TGTACTTCGTCCCAGGGTGTGTGAG 3'
      S235' ACTCCGCCATGAATCACTCCCCT 3'
      R52395' GCCCAAACGGTACAGGAGAGGTG 3'
      S46755' GACGGGGTACACTGGAGACTTTGA 3'
      R94405' ACCGAGCGGGGAGTAGGAAGAGG 3'
      S47415' CTTCAGCCTGGACCCCACCTTC 3'
      R93985' GGAGTAGGCCGAAGAGTAATGAGC 3'
      EI/AvrII-S5' ATTCCTAGGCCTCTCCCTCCCCCCCCCCTAACGTT 3'
      EI/NS3-R5' GGGGGCCATGGTATCATCGTGTTTTTCAAA 3'
      EI/NS3-S5' CACGATGATACCATGGCCCCCATCACTGCT 3'
      NS3/KpnI-R5' GGCGGTACCCAACCTGGTAGGTCTGGGGC 3'
      Luc-S5' AACCGTCGCCCACCATGGAAGACGCCAAAAACATA 3'
      Luc-A5' GCCGTTTAAACTTACACGGCGATCTTTCCGCCCTT 3'
      J/C7680-s5' CCTGGACCGGGGCTCTAATAACTC 3'
      J/C7703-a5' GAGTTATTAGAGCCCCGGTCCAGG 3'
      J/C6248-s5' GAGGACTGCCCCATCCCATGC 3'
      J/C6268-a5' GCATGGGATGGGGCAGTCCTC 3'
      J/C5325-s5' GCACGTGGGTCCTAGCTGGAGGAGTC 3'
      J/C5350-a5' GACTCCTCCAGCTAGGACCCACGTGC 3'
      J/C5459-s5' GAGGCTTTTGATGAGATGGAGGAATG 3'
      J/C5484-a5' CATTCCTCCATCTCATCAAAAGCCTC 3'
      J/C4127-s5' GCTGCCACCCTGGGGTTTGGGGCG 3'
      J/C4150-a5' CGCCCCAAACCCCAGGGTGGCAGC 3'
      J/C5025-s5' GGGAGGCAGTTTTCACCGGCC 3'
      J/C5045-a5' GGCCGGTGAAAACTGCCTCCC 3'
      J/C8971-s5' CCAAGACACCCTGGACCAGAACC 3'
      J/C8993-a5' GGTTCTGGTCCAGGGTGTCTTGG 3'
      CN3-P29Q-s5' GCGACAAGACCGAACAGGCCGGGGAAATCC 3'
      CN3-P29Q-a5' GGATTTCCCCGGCCTGTTCGGTCTTGTCGC 3'
      CN3-P47T-s5' CTTTCCTCGGAACGACTATTTCGGGGG 3'
      CN3-P47T-a5' CCCCCGAAATAGTCGTTCCGAGGAAAG 3'
      CN3-D71V-s5' CACGGGGTCCGGTCACACAGATGTACTC 3'
      CN3-D71V-a5' GAGTACATCTGTGTGACCGGACCCCGTG 3'
      CN3-N185S-s5' GGTCTCCCACCTTCAGTGACAACAGCTCACC 3'
      CN3-N185S-a5' GGTGAGCTGTTGTCACTGAAGGTGGGAGACC 3'
      CN3-R246H-s5' CTTGTCCAAGGCACATGGCATCAACCC 3'
      CN3-R246H-a5' GGGTTGATGCCATGTGCCTTGGACAAG 3'
      CN3-D281S-s5' GATGGGGGCTGTGCAAGCGGCGCCTATGACG 3'
      CN3-D281S-a5' CGTCATAGGCGCCGCTTGCACAGCCCCCATC 3'
      CN3-T347I-s5' CAGGAGGGCGAGATCCCCTTCTATGGG 3'
      CN3-T347I-a5' CCCATAGAAGGGGATCTCGCCCTCCTG 3'
      CN3-S497A-s5' GTGAGTGCTACGACGCAGGGGCTGCATGG 3'
      CN3-S497A-a5' CCATGCAGCCCCTGCGTCGTAGCACTCAC 3'
      C5B-T35N-s5' GCTGTTGCGATACCACAACAAGGTGTACTGTACTAC 3'
      C5B-T35N-a5' GTAGTACAGTACACCTTGTTGTGGTATCGCAACAGC 3'
      C5B-A179T-s5' GGCCCTTTATGATGTCACACAAAAGCTCCCTC 3'
      C5B-A179T-a5' GAGGGAGCTTTTGTGTGACATCATAAAGGGCC 3'
      C5B-A322V-s5' GCGACGACTTGGTTGTCATCTCAGAGAGCC 3'
      C5B-A322V-a5' GGCTCTCTGAGATGACAACCAAGTCGTCGC 3'
      C5B-E310A-s5' GCAGGGATAGTTGCGCCCACGATGCTG 3'
      C5B-E310A-a5' CAGCATCGTGGGCGCAACTATCCCTGC 3'
      C5B-V405I-s5' CTAGACACTCCCCTATCAATTCATGGCTGG 3'
      C5B-V405I-a5' CCAGCCATGAATTGATAGGGGAGTGTCTAG 3'
      J5166-s5' ACGCCATGTGGAAGTGCCTGGCCCG 3'
      C4452-a5' AGGGCTACCTCCTCTATATTGGGG 3'
      C4220-s5' CCCATCACATACTCCACATATGGC 3'
      C8486-a5' AGGTCTGACCCTTGCTGTTAAACATGG 3'
      C8477-s5' GGTCAGACCTGCGGGTACAGGCGTTG 3'
      M13-R5' CAGGAAACAGCTATGACC 3'

      Table 1.  Primers used in this study

    • Amino acid residue (JFH1) CCH JFH1 Con1 H77
      NS3 1059 P Q V V
      1077 P T C C
      1101 D V I V
      1215 N S T T
      1276 R H H H
      1311 D S G G
      1377 T I I I
      1527 S A A A
      NS5B 2477 T N N N
      2621 A T V V
      2752 E A D D
      2764 A V V V
      The CCH NS3 and NS5B sequences were searched against the HCV Sequence Database, and a number of CCH unique sites that differed from all of the reference sequences were revealed. Amino acid residue numbers refer to the JFH1 reference sequence (AB047639).

      Table 2.  Sequence analysis of CCH NS3 and NS5B